Investigation on the Acute and Subacute Toxicity of Alhagi Graecorum in Experimental Animals.
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Date
2013-07
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Abstract
Little is known about the acute and subacute toxicity of Alhagi Graecorum (Camel Thorn) in mice. The
aim of this study is to investigate the acute toxicity (LD50) and sub acute toxicity of camel thorn in mice. For
determination of LD50, mice weighing 25-30 g were divided into nine groups each of 6 animals and received 130, 330,
660, 1300, 2600, 4200, 5000, 5900 and 6900 mg/kg orally of camel thorn water extract respectively. The sign and
symptoms of toxicity and the number of died animals in each group were registered and the LD50 was
calculated.Another group of Male albino mice weighed 25-30 g were divided into control and different treated group
each of 6 mice and placed in the metabolism cages that allowed daily measurement of food and water consumption. The
control received normal saline whereas the other groups received 130, 660, 1300 mg/kg (i.p, daily) of camel thorn
extract (CTE)respectively, for two weeks.. The animals were weighed daily for any sign of reduction or gain of body
weight. The food and water consumption were daily calculated. At the end of this experiment, the mice were killed and
blood was collected and used for the determination of glucose and the serum creatinine,urea, aspartate,
aminotransferase, (AST) and alanine aminotransferase (ALT) levels.The liver of the animals were removed,fixed in
10%formaline for histopathological investigation. The LD50 was equal to 5400mg/kg.Results of sub acute toxicity
studies revealed that no significant weight reduction were observed in treated groups as compared to control, however
the food consumption was significantly increased in the mice received 660mg/kg of CTE . Also the water consumption
was significantly decreased in the animal receiving 1300mg/kg of CTE.
Description
Keywords
Alhagigraecorum, camel thorn, acute toxicity, subacute toxicity
Citation
Gargum Huda, Zoki Abd El-Naser Al, El-Barassi Idris F, Muftah Saleh Saeed, Shaari Faraj El, Jumma Masaud, Elzagheid Adam, Abdellatif Awad G. Investigation on the Acute and Subacute Toxicity of Alhagi Graecorum in Experimental Animals. International Journal of Applied Biology and Pharmaceutical Technology. 2013 Jul-Sept; 4(3): 202-209.