Improvement Germination and Conversion of Somatic Embryos and Production of Normal Plantlets in Ferula Assa-foetida L. (A Rare Medicinal Plant).
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Date
2014-12
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Abstract
Secondary somatic embryogenesis leads to the formation of abnormal somatic embryos
and produces abnormal seedlings. Normal plants are difficult to obtain from these
embryos, due to the asynchronous maturation of the embryogenic tissues and low
germination and conversion rates. The effects of some media additives and different
strengths of MS medium on germination and plantlet formation of in vitro derived somatic
embryos of Ferula Assa-foetida were studied. The highest number of normal embryos
was observed in MS medium containing 30g/l sucrose with 0.5% or 1% AC and in MS
medium supplemented with PEG and 0.5% or 1% AC. The treatments of MS medium with
30g/l sucrose and 0.5% AC × MS medium containing sorbitol and MS medium containing
PEG and 1% AC × ½ MS had maximum number of normal germinated embryos without
secondary somatic embryogenesis (SSE). In some of the treatments the embryos were
converted better than the others, such as; the interaction effect of MS medium with 30g/l
sucrose and 0.5% AC× MS, MS medium with 30g/l sucrose × MS medium with glutamine.
Using different strength of MS medium and presence of some media additives is effective
on germination and conversion of somatic embryos into normal plantlet. Presence of
Activated Charcoal in the culture medium can reduce secondary somatic embryogenesis.
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Keywords
Somatic embryo germination, conversion, manitol, polyethylene glycol, Activated, Charcoal (AC)
Citation
Roozbeh Shirin, Otroshy Mahmoud. Improvement Germination and Conversion of Somatic Embryos and Production of Normal Plantlets in Ferula Assa-foetida L. (A Rare Medicinal Plant). European Journal of Medicinal Plants. 2014 Dec; 4(12): 1447-1461.