In vitro Antisickling and Radical Scavenging Activities of a Poly-herbal Formula (Drepanoalpha®) in Sickle Cell Erythrocyteand Acute Toxicity Study in Wistar Albino Rats.
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Date
2014-10
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Abstract
Aims: To evaluate the antisickling and radical scavenging activities and acute toxicity of
indigenous nutritive formula Drepanoalpha®, produced through a bio-guided based plant
selection.
Study Design: Drepanoalpha® extracts, Antisickling activity by Emmel test, Antioxidant
activity by 1,1-diphenyl-2-picrylhydrazyl bleaching methods; acute toxicity on rats,
determination of biological and haematological parameters.
Place and Duration of Study: Science Faculty University of Kinshasa, between January
2013 and February 2014.
Methodology: The antisickling and antioxidant activities of Drepanoalpha® were
determined using Emmel and the 1,1-diphenyl-2-picrylhydrazyl bleaching methods respectively. Acute oral toxicity test was performed to determine the LD50. Liver and
kidney functions, the hematological and histopathological examinations were assessed
using standard techniques.
Results: Obtained results revealed that Drepanoalpha® possessesinteresting in vitro
antisickling and antioxidant activities as revealed by the observed normal biconcave form
of sickle erythrocyte (normalization rate >80%) and the radical scavenging activity (ED50=
0.604 ± 0.028 μg/mL). Acute toxicity assessment revealed that the medium lethal dose
(LD50) is higher than 4000 mg/kg. Drepanoalpha® significantly increases the values of
WBC, RBC, Hb, HCT, PLT, IDR-CV and PCT. Furthermore, this polyherbal formula
significantly decreases the values of IDR-SD, P-RGC, AST and ALT (p<0.05). Both the
control and treated groups displayed comparable non altered histological architecture of
the liver cells.
Discussion: The mean values of biochemical markers and hematological markers of
treated rats revealed that Drépanoalpha® is potentially safe indicating non-toxic effect of
the phytomedicine on immune cells and blood clotting factors. Moreover, this poly-herbal
formulation increases the hemoglobin rate in the all treated rats (500-4000 mg/kg
bodyweight) and preserves the histological architecture of the liver cells.
Conclusion: Drepanoalpha® may increase weight gain, promote erythropoiesis and
thrombopoeisis in sicklers patients. This phytomedicine could be used in the treatment of
all form of anemia and may also prevent bile duct obstruction or intra-hepatic cholestasis.
The results can form the basis for clinical trials in humans.
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Keywords
Sickle cell disease, Drepanoalpha®, DL50, wistar albino rat, anthocyanins
Citation
Ngbolua K N, Mpiana P T, Tshibangu D S T, Mazasa P P, Gbolo B Z, Atibu E K, Kadima J N, Kasali F M. In vitro Antisickling and Radical Scavenging Activities of a Poly-herbal Formula (Drepanoalpha®) in Sickle Cell Erythrocyteand Acute Toxicity Study in Wistar Albino Rats. European Journal of Medicinal Plants. 2014 Oct; 4(10): 1251-1267.