Antiproliferative Effect of Portlandia Extracts and Their Fractions on Breast Cancer Cells.
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Date
2014-10
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Abstract
Aim: Rubiaceae is one of the largest families of plants and it includes the Jamaican genus
Portlandia. This family has been used in several ethnomedicinal practices. Thus, in this
study we investigated whether extracts of two species of Portlandia affect In vitro
proliferation of breast cancer cells.
Study Design: Metastatic (MDA-MB-231 and HeLa) cells were incubated in the absence
or in the presence of Portlandia extracts. A human non-metastatic (MCF-10A) and normal
(ATCC PCS-600-010) epithelial cell lines were used as control.
Methodology: At the end of the study all cell lines were incubated with Portlandia extracts
(-and its subfractions-) and then analyzed on proliferation, thymidine incorporation and
mitogenic and survival signaling pathways on metastatic and non-metastatic cells.
Results: It was found that Portlandia methanol extract inhibited proliferation of MDA-MB-
231 in a dose-dependent manner attenuating the phosphorylation of Erk1/2 and Akt1 without affecting the proliferation of MCF-10A cells. [3H] thymidine incorporation was also
decreased by Portlandia methanol extract. This study suggests that Portlandia extracts
selectively affected the proliferation of metastatic breast cancer cells through the
modulation of Erk1/2 and Akt1 activities that play a critical role during cell survival and
proliferation.
Conclusion: Portlandia leaf extracts contain active compounds, which strongly repress
cancer cell proliferation in a dose-dependent manner and selectively down-regulate
phosphorylation of both Erk1/2 and Akt1 activities.
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Keywords
Portlandia, breast cancer cells, proliferation, signaling transduction pathways
Citation
Chin C, Veisaga M L, Priestap H A, Commock T, Campbell K, Jestrow B, Francisco-Ortega J, Barbieri M A. Antiproliferative Effect of Portlandia Extracts and Their Fractions on Breast Cancer Cells. European Journal of Medicinal Plants. 2014 Oct; 4(10): 1186-1199.