In vitro Modulation of Pancreatic Insulin Secretion and Extra Pancreatic Insulin Action, Enzymatic Starch Digestion and Protein Glycation by Terminalia chebula Extracts.
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Date
2014-07
Authors
Kasabri, V
Flatt, P R
Abdel-Wahab, Y H A
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Abstract
Aim: Traditional plant treatments have been used throughout the world for the therapy of
diabetes mellitus.
Study Design: Using multiple in vitro models; this study was designed to investigate the
efficacy and mode of action of Terminalia chebula Retz. (Combretaceae) used
traditionally for treatment of diabetes.
Place and Duration of Study: School of Biomedical Sciences, University of Ulster, 2001-
2004.
Results: T. chebula aqueous extract stimulated basal insulin output and potentiated
glucose-stimulated insulin secretion concentration-dependently in the clonal pancreatic
beta cell line, BRIN-BD11 (p<0.001). The insulin secretory activity of plant extract was
abolished in the absence of extracellular Ca2+ and by inhibitors of cellular Ca2+ uptake,
diazoxide and verapamil, (p<0.001). Furthermore, the extract increased insulin secretion
in depolarised cells and augmented insulin secretion triggered by IBMX, but not by
tolbutamide or glibenclamide. T. chebula extract did not display insulin mimetic activity but it enhanced insulin-stimulated glucose transport in 3T3 L1 adipocytes by 280% (p<0.001).
At (0.5-5.0mg/mL) concentrations, the extract also produced 22-84% (p<0.001) decrease
in starch digestion In vitro and inhibited protein glycation (p<0.001) at 1mg/ml aqueous
extract.
Conclusion: This study has revealed that water soluble bioactive principles in T.chebula
extract stimulate insulin secretion, enhance insulin action and inhibit both protein glycation
and starch digestion. The former actions are dependent on the bioeffective component(s)
in the plant being absorbed intact. Future work assessing the use of Terminalia chebula
as dietary adjunct or as a source of active antidiabetic agents may provide new
opportunities for the treatment of diabetes
Description
Keywords
Terminilia chebula Retz (Combretaceae), insulin secretion, insulin action, starch digestion, peptide glycation
Citation
Kasabri V, Flatt P R, Abdel-Wahab Y H A. In vitro Modulation of Pancreatic Insulin Secretion and Extra Pancreatic Insulin Action, Enzymatic Starch Digestion and Protein Glycation by Terminalia chebula Extracts. European Journal of Medicinal Plants. 2014 Jul; 4(7): 771-782.