Phytochemical Evaluation and Effect of Saponins’ Mixture Isolated from Astragalus monspessulanus on HepG2 Cell Line.
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Date
2014-05
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Abstract
Aims: Purified saponin fraction was obtained from butanol extract of the aerial parts of
Astragalus monspessulanus var. monspessulanus. The fraction was analyzed by HPLC
and six saponins were determined. The saponin mixture was investigated for possible
cytotoxicity on HepG2 cell line.
Study Design: HPLC, in vitro study, biochemical analysis - lactate dehydrogenase
leakage in medium
Place and Duration of Study: Laboratory of Drug metabolism and drug toxicity, between
September and November 2013.
Methodology: The saponin mixture was studied on HepG2 cell line in 3 different
concentrations –1, 2, 4 mg/ml for 24, 48 and 72 hours. The release of lactate
dehydrogenase in the medium was measured spectrophotometrically.
Results: It was found that the saponins have statistically significant cytotoxicity only in the highest concentration 4 mg/ml on 48 and 72 hours. At this concentration the sample
increased the level of LDH (lactate dehydrogenase) with 37 % on 48 h and with 52 % on
72 h.
Conclusion: Purified saponin fraction, isolated from Astragalus monspessulanus was
found to be cytotoxic at the highest concentration 4 mg/ml on 48 and 72 hours in HepG2
cell line.
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Keywords
Astragalus monspessulanus, saponins, LDH leakage, HepG, cytotoxicity
Citation
Bratkov Viktor, Kondeva-burdina Magdalena, Simeonova Rumyana, Tzankova Virginia, Krasteva Ilina. Phytochemical Evaluation and Effect of Saponins’ Mixture Isolated from Astragalus monspessulanus on HepG2 Cell Line. European Journal of Medicinal Plants. 2014 May; 4(5): 522-527.