Pharmacological Activities of Agave seemanniana and Isolation of Three Steroidal Saponins.
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Date
2014-03
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Abstract
Aim: To investigate the analgesic, anti-inflammatory and ulceroprotective properties of
Agave seemanniana methanolic leaf extract (MEAS) and its saponin containing fraction
(SFAS), in animal models compared to standard drugs. In addition to separation and
characterization of the major saponin fraction of Agave seemanniana leaves.
Place and Duration of Study: The study was carried out at the Department of
Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt, between June
2011 and March 2013.
Methodology: The analgesic activity was assessed using the hot plate method and
aspirin being a positive standard, the anti-inflammatory activity was investigated using the
standard carrageenan-induced paw edema method against indomethacin as standard
and the ethanol induced ulcer was used to record the ulcer protective effect of methanolic
leaf extract of Agave seemanniana (MEAS) and saponin fraction of Agave seemanniana (SFAS). Separation and characterization techniques were used for the phytochemical
study of the saponin content of the extract.
Results: The results of analgesic activity of MEAS and SFAS using the hot plate test,
revealed significant increase in the reaction time at dose of 100mg/kg b.wt. The same
dose of the two test drugs demonstrated significant ulcer protective activity. The results of
anti-inflammatory activity revealed that the reduction of the carrageenan-induced paw
edema was significant at a dose of 100mg/kg b.wt. Furthermore, SFAS contained mainly
saponins of pregnane and furostanol types. The structures of three isolated and
characterized saponins indicated that these saponins had a common oligosaccharide
moiety linked to the aglycone C-3 position and identified as α-L- rhamnopyranosyl–
(1→4)-β-D-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl- (1→4)-
β-D-galactopyranosyl.
Conclusion: This study contributes to the search for potent and locally available plant
materials as analgesic, anti-inflammatory and ulcer protective drugs known for their high
saponin content.
Description
Keywords
Agave seemanniana, Analgesic, Anti-inflammatory, Ulceroprotective
Citation
Mina S A, Melek F R, Abdel-khalik S M, El-Shaarawy F S, Eskander Jacqueline. Pharmacological Activities of Agave seemanniana and Isolation of Three Steroidal Saponins. European Journal of Medicinal Plants. 2014 Mar; 4(3): 271-283.