Biological Activities of the Hydro-alcoholic and Aqueous Extracts of Achillea falcata L. (Asteraceae) Grown in Jordan.
Loading...
Date
2014-03
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aim: This study aimed to screen the aqueous and hydro-alcoholic extracts of Achillea
falcata L. (Asteraceae) grown in Jordan for their antioxidant, antibacterial, antiplatelet and
anti-proliferative efficacy.
Study Design: HPLC-MS evaluation of the aqueous and hydro-alcoholic extracts and in
vitro investigations.
Place and Duration of Study: Faculties of Pharmacy and Science, The University of
Jordan and Centre of Misanalysis, National Institute for Biological Sciences, between
August 2012 and June 2013.
Methodology: Total phenols and flavonoids were determined colorimetrically. The radical scavenging activities were evaluated using 2,2′-azino-bis-3-ethylbenzthiazoline-6-
sulphonic acid (ABTS) radical scavenging activity assay. Antimicrobial activities were
determined by the disc-diffusion method, and the minimum inhibition concentration and
the minimum bactericidal concentration tests. In vitro antiplatelet activity was tested on
human whole blood using an electrical impedance method. Anti-proliferative activity was
investigated using the MTT assay. High performance liquid chromatography-mass
spectrometry (HPLC-MS) evaluation was performed.
Results: Hydro-alcoholic extract had a bactericidal activity against Streptococcus
pneumoniae, Bacillus cereus and Klebsiella pneumoniae rather than inhibitory effect. No
significant activity was observed against gram negative bacteria and Candida albicans. In
vitro antiplatelet activity was tested on human whole blood using an electrical impedance
method. At concentrations (50, 100, and 200 μg/ml), hydro-alcoholic extract did not show
effect on platelet aggregation. Extracts did not possess cytotoxic activity against the MCF-
7 cells at concentrations up to 200 μg/ml. HPLC-MS analysis resulted in the identification
of 8 phenolic compounds in the hydro-alcoholic extract and 6 compounds in the aqueous
extract; quercetin 3-β-D-glucoside was the main component for both extracts.
Conclusion: The present investigation supported the traditional use A. falcata in the
Jordanian folk medicine as a depurative agent and as an antimicrobial active
representative of the genus Achillea.
Description
Keywords
Achillea falcate, Asteraceae, Antioxidant activityAntioxidant activity, Antiplatelet activity, Cytotoxicity, Jordan
Citation
Hammad Hana M, Litescu Simona-Carmen, Matar Suzan A, Al-Jaber Hala I, Afifi Fatma U. Biological Activities of the Hydro-alcoholic and Aqueous Extracts of Achillea falcata L. (Asteraceae) Grown in Jordan. European Journal of Medicinal Plants. 2014 Mar; 4(3): 259-270.