Microtuberization of Ceropegia pusilla Wight and Arn. an Endangered Medicinal Plant.
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Date
2014-01
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Abstract
An efficient micropropagation (direct & indirect) and microtuberization protocol for an
endemic and ethanomedicinal plant Ceropegia pusilla is described. The highest number
of shoot initiation (3.50 0.34) and higher average shoot number in shoot subculture
(15.40 1.65) were recorded on MS medium supplemented with BAP+NAA (2.5+0.5
mg/l). The best performance of callus induction and morphogenesis was found on MS
medium supplemented with BAP and NAA from stem. The callus initiation was recorded
on MS medium supplemented with BAP+NAA (0.5 + 1.0 mg/l) and the higher percentage
of callus forming shoots from callus subculture is determinate to be 90-95 7.93. MS
medium supplemented with various concentrations of cytokinins and auxins supported the
induction of microtubers, in vitro flowering and roots. The microtubers and root formation
occurred on the basal region of the shoots in MS medium containing BAP (1.5mg/l) and
NAA (0.5mg/l). The higher average number of root length was (2.8 0.68cm) also
observed in the same concentration. The plantlets regenerated in vitro with welldeveloped
shoots, microtubers and roots were successfully established in pots containing hardening media and grown in a shade house with 81 3.16% survival rate.
Description
Keywords
Microtubers, Ceropegia pusilla, basal tubers, medicinal plants
Citation
Kalimuthu K, Prabakaran R, Paulsamy S, Jeyaraman S. Microtuberization of Ceropegia pusilla Wight and Arn. an Endangered Medicinal Plant. European Journal of Medicinal Plants. 2014 Jan; 4(1): 64-74.