Identification of cis- and trans- Melilotoside within an Artemisia annua Tea Infusion.
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Date
2014-01
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Abstract
Aims: To identify the major chemical components contained within the Artemisia annua
tea infusion, which has been traditionally used to treat fevers and chills.
Study Design: Currently, little chemical data exists on the tea infusion as previous work
only focused on LC-MS analysis and although a valuable analytical tool it remains difficult
to identify new compounds. Our study therefore employed LC-MS and NMR analysis in
order to confirm the identities of the major compounds.
Place and Duration of Study: Natural Products Laboratory, Institute of Biology, Leiden
University between Jan 2012 and December 2012.
Methodology: A thorough chemical analysis of the tea infusion was completed using
both LC-MS and NMR analyses. Tea infusions were prepared using deionised water and
were subjected to LC-MS analysis followed by semi-preparative fractionation and NMR
analysis to confirm the identities of the major compounds.
Results: Eleven major compounds were identified including chlorogenic acids,
feruloylquinic acids, flavonols, coumarins, of which two compounds, cis- and transmelilotoside,
are new for Artemisia spp.
Conclusion: The melilotosides are known to be active against diarrhoea causing
pathogens and therefore might explain the traditional use of A. annua to treat diarrhoea.
Future work will focus on the quantification of the melilotosides and the identification of other compounds (major and minor) in the tea infusion.
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Keywords
Artemisia annua, tea infusion, malaria, melilotosides, HIV, cancer
Citation
Mouton J, Kooy F Van der. Identification of cis- and trans- Melilotoside within an Artemisia annua Tea Infusion. European Journal of Medicinal Plants. 2014 Jan; 4(1): 52-63.