Antioxidant Enzymes Activity and Hormonal Changes Following Administration of Ethanolic Leaves Extracts of Nauclea latifolia and Gongronema latifolium in Streptozotocin Induced-Diabetic Rats.
Loading...
Date
2013-04
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aim of the Study: To evaluate the effects of ethanolic leaf extracts of Gongronema
latifolium (G.L) and Nauclea latifolia (N.L) on antioxidant enzymes activity (GPx, SOD and
CAT) and hormonal status (T3, T4, Insulin, c-peptide) in streptozotocin-induced diabetic
Wistar rats.
Material and Methods: Thirty six (36) albino Wistar rats of both sexes weighing 150-250g
were divided into 6 groups of 6 rats each. Groups 1, 2 and 3 received 400mg/kg body weight (b.w) of G.L, N.L and 200mg/kg b.w each of G.L and N.L respectively while group
4 received 5 iu/kg b.w of insulin subcutaneously daily for 21 days, Groups 5 and 6 served
as controls (diabetic and Normal) and received placebo. Fasting blood glucose was
determined at the start of the experiment and thereafter at 72 hours interval and at the
end of experimental period. The animals were sacrificed and sera preparations were used
for antioxidant enzymes and hormonal assays.
Results: Blood glucose in diabetic animals decreased significantly (P=.05) by 66.34%,
18.12%, 67.73% and 86.62% of initial values upon treatment with G.l, N.l, G.I plus N.I and
insulin respectively. There was only a 24.44% decrease in the diabetic control. A
significant decrease (P=.05) in insulin and T3 levels was observed in the diabetesinduced
rats (65 and 85% respectively) compared to NC. The levels of the hormones
where however significantly increased (P=.05) on treatment of the diabetic animals with
G.l, N.l, G.I plus N.I and insulin. Whereas a significant decrease (P=.05) was observed in
T4 level of DC rats compared to the NC, treatment with the leaf extracts and insulin did
not result in any elevation of the hormone relative to DC. The C-peptide levels for all
groups were much lower than the corresponding insulin levels, suggesting a type 1
diabetes in the diabetes-induced rats. A significant decrease (P=.05) in activity was
observed for GPx and SOD in the DC group relative to NC. A combination of G.l and N.l
gave a much higher reversal in activity (P<.01) when compared to treatments with
individual leaf extracts. There was a significant increase (P=.05) in CAT activity in the DC
animals relative to NC. This was potentiated in all treatment groups with the combination
group showing a synergy in its potentiating effect.
Conclusion: There was a reversal in the level of the hormones and the activity of the
antioxidant enzymes towards normal control, and comparable to the reversals by
treatment with insulin, on treatment of the diabetic animals with the leaves extracts
especially in combination. The results taken together indicate a synergy that makes the
combination of the two plants extracts a potent antidiabetic remedy.
Description
Keywords
Diabetes mellitus, Gongronema latifolium, Nauclea latifolia, hormonal indices, antioxidant enzymes, hyperglycemia, combined extract
Citation
Effiong Grace S, Mgbeje Bob IA, Igile Godwin O, Atangwho Justin I, Eyong Eyong U, Ebong Patrick E. Antioxidant Enzymes Activity and Hormonal Changes Following Administration of Ethanolic Leaves Extracts of Nauclea latifolia and Gongronema latifolium in Streptozotocin Induced-Diabetic Rats. European Journal of Medicinal Plants. 2013 Apr-Jun; 3(2): 297-309.