Antihemolytic Activity of Clerodendrum viscosum Vent. is Mediated by its Antioxidant Effect.
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Date
2013-01
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Abstract
Aims: The objective of this study was to evaluate the protective effect of polar extract of
Clerodendrum viscosum vent. against in vitro hemolysis of human erythrocytes and its
association with the antioxidant activity of C. viscosum.
Study Design: Extraction of C. viscosum dried root, in vitro antihemolytic activity assay,
lipid peroxidation assay, phytochemical analysis, estimation of polyphenols and
flavonoids.
Place and Duration of Study: Department of Biochemistry and Molecular Biology,
Jahangirnagar University, Savar, Dhaka, Bangladesh, between January 2011 and May
2011.
Methodology: C. viscosum polar extract-pretreated erythrocytes were hemolysed by
hypotonia and oxidizing agent, H2O2. The liberated hemoglobin was determined as a
measure of hemolysis. Total reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-
free radical scavenging activity of extract were compared with those of vitamin C. Anti- lipid peroxidation activity of C. viscosum polar extract also was determined by exposing
rat brain cortex tissue to Fenton's reagent (H2O2+FeSO4)- induced oxidative stress. Then
C. viscosum extract was subjected to estimation of total polyphenols and total flavonoids
following qualitative phytochemical analysis.
Results: C. viscosum polar extract significantly inhibited in vitro hemolysis. Total reducing
power and DPPH-radical scavenging activity were higher than those of vitamin C. In
cortical tissue homogenate, C. viscosum polar extract significantly reduced (~38%) the
levels of lipid peroxides (LPO). Phytochemical analysis revealed the presence of
substantial amount of polyphenols, flavonoids and other antioxidant chemicals in the
extract.
Conclusion: Present investigation demonstrates that antihemolytic activity of C.
viscosum polar extract is mediated by its antioxidant effect.
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Keywords
Hemolysis, lipid peroxidation, C viscosum, antioxidant.
Citation
Rahman Mijanur, Rahaman Asiqur, Basunia Mafroz Ahmed, Fatima Nusrat, Hossain Shahdat. Antihemolytic Activity of Clerodendrum viscosum Vent. is Mediated by its Antioxidant Effect. European Journal of Medicinal Plants. 2013 Jan-Mar; 3(1): 127-134.