Cytoprotective Potential of Royal Jelly on Human Umbilical Vein Endothelial Cells against Nicotine Toxicity via Catalase.
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Date
2013-01
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Abstract
Aims: To examine the cytoprotective effects and mechanisms of a royal jelly extract in
protecting the human umbilical vein endothelial cells (HUVECs) from nicotine toxicity.
Study Design: Laboratory experimental tests.
Place and Duration of Study: Department of Physiology and Department of Surgery,
Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand, between June
2011 and February 2012.
Methodology: Cytotoxic assay of royal jelly to HUVECs was performed by using the 3-
(4,5-dimethylthiazol,2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent. The
cytoprotective effect was then investigated by examining the presence of vacuole-like
structures in HUVECs exposed to nicotine 5 or 7.5 mM with and without royal jelly. Cells
were stained with crystal violet and photographed under phase contrast microscope.
mRNA levels of genes involved in intracellular antioxidant system, superoxide dismutase
(SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GSR)
were confirmed by reverse transcription-polymerase chain reaction (RT-PCR). Catalase activity was also determined by examining peroxidative function.
Results: Vacuole-like structures were found in the cytoplasm of HUVECs exposed to 5
mM nicotine and higher. Royal jelly alone at the concentrations lower than 2 mg/ml did
not affect the structure or the survival rate of HUVECs after 1, 4, and 7 days of treatment.
For cytoprotective effect, royal jelly 1-4 mg/ml mixed with 5 mM nicotine could obviously
decrease the numbers of cells containing vacuole-like structures in the cytoplasm of
HUVECs with the dose- and time-dependent fashion. The catalase mRNA levels and
catalase activity in HUVECs exposed to 5 mM nicotine decreased significantly, but
recovered when the cells were treated with royal jelly.
Conclusion: Royal jelly can be safety applied to endothelial cells even at high doses.
Royal jelly is able to attenuate the abnormal vacuole-like structures induced in endothelial
cell cytoplasm when exposed to nicotine. Further investigation of antioxidant gene
expression showed that the mechanism possibly involves a reduction of oxidative stress
via an up-regulation of catalase. Besides the supplementary food, royal jelly could be
useful for endothelial cell protection from nicotine toxicity found in smoking or nicotine
addiction.
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Keywords
Royal jelly, protective effect, nicotine toxicity, antioxidant, catalase
Citation
Supabphol Roongtawan, Supabphol Athikom. Cytoprotective Potential of Royal Jelly on Human Umbilical Vein Endothelial Cells against Nicotine Toxicity via Catalase. European Journal of Medicinal Plants. 2013 Jan-Mar; 3(1): 88-98.