Spectrophotometric determination of ritonavir by condensation method using ninhydrin and ascorbic acid.
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Date
2013-01
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Abstract
A simple and sensitive extractive visible spectrophotometric method for the assay of Ritonavir in pure
and pharmaceutical Formulations based on the reaction between peptide group in RIT and Ninhydrin in the presence
of ascorbic acid affords a blue violet coloured product (λ max 560nm). Regression analysis of Beer-Lambert plots
showed good correlation in the concentration ranges (20-60) μg/ml. The percent recoveries are obtained as 99.64 ±
0.47 to 100.40 ± 0.45 by proposed method and 99.51 ± 0.25 to 99.92 ± 0.20 by reference method for the
formulations respectively. The method can be applied successfully for the estimation of the Ritonavir in the presence
of other ingredients that are usually present in formulations. The method offers the advantage of rapidity, simplicity
and sensitivity and low cost without the need for expensive instrumentation and reagents.
Description
Keywords
Statistical analysis, Ritonavir(RIT), Ascorbic Acid, Ninhydrin, Peptide group
Citation
Acharyulu M L N, Murthy Ch V R, Reddy T S, Prasad S V Maruti, Ashok S. Spectrophotometric determination of ritonavir by condensation method using ninhydrin and ascorbic acid. International Journal of Applied Biology and Pharmaceutical Technology. 2013 Jan-Mar; 4(1): 53-57.