DNA binding and cytotoxicity of newly synthesized schiff base (z)-4-(((2-hydroxy phenyl) amino) (phenyl) methylene)-3-methyl-1-phenyl-1hpyrazol-5(4H)-one and its analogues.
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Date
2012-10
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Abstract
A novel Schiff base (Z)-4-(((2-hydroxy phenyl)amino)(phenyl)methylene)-3-methyl-1-phenyl-1Hpyrazol-
5(4H)-one and its analogues were synthesized by condensation of 5-methyl-2-phenyl-4-substituted pyrazolin-
3-one with 2-amino phenol from alcoholic solution.Schiff base ligands arecharacterizedby IR, UV, NMR, elemental
analysis and single crystal X- ray diffraction analysis. These potential ligands are subjected to DNA binding analysis
against the Calf thymus DNA, their binding constant values were calculated and compared to the standard ruthenium
intercalators. The cytotoxic natureof these Schiff base ligands was also studied with the human breast cancer cell line
MCF-7 and their IC50 values were determined.
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Keywords
Pyrazole Schiff bases, Tautomerism, DNA binding, Cytotoxicity, IC50
Citation
Gowri M, Jayabalakrishnan C. DNA binding and cytotoxicity of newly synthesized schiff base (z)-4-(((2-hydroxy phenyl) amino) (phenyl) methylene)-3-methyl-1-phenyl-1hpyrazol-5(4H)-one and its analogues. International Journal of Applied Biology and Pharmaceutical Technology. 2012 Oct-Dec; 3(4): 327-337.