Analysis of methyl parathion in biological samples using thin layer chromatography.
Loading...
Date
2012-07
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Pesticides are major contaminating chemicals in agriculture environment and a hazard to
exposed population. The pesticides form a strong class of environment pollutants, as they are sometimes
nonbiodegradable, damaged not only the environment and agriculture but also have entered into the food chain
thereby affecting health and development. Methyl parathion is a broad-spectrum organophosphorus insecticide
generally used to control a variety of insects. The present study was planned to develop a new method for
analysis of Methyl parathion in human blood samples using thin layer chromatography technique, which is
simple and quick. Methyl Parathion was extracted from blood using solvent extraction methods and then
identified on the TLC plates. For chromatographic separation, various binary and tertiary solvent systems were
used and for detection on developed plates, palladium chloride reagent was used which successfully increased
the sensitivity without dispensing with the simplicity of the method. Statistical analysis was performed on four
solvent systems namely benzene: chloroform (80:20), hexane: carbontetrachloride (50:50), hexane:
carbontetrachloride (20:80), hexane: propanol (20:80) which included the calculation of mean Rf value, value
of standard deviation and coefficient of variance. It is evident from the statistical data that hexane:
carbontetrachloride (50:50), hexane: carbon tetrachloride (20:80) is preferably good solvent systems for
parathion identification by thin layer chromatography.
Description
Keywords
Organophosphorous compound, Methyl parathion, Rf, Palladium chloride
Citation
Kumawat Ginni, Swati, Karnawat Romila, Sharma I K, Verma P S. Analysis of methyl parathion in biological samples using thin layer chromatography. International Journal of Applied Biology and Pharmaceutical Technology. 2012 Jul-Sept; 3(3): 203-209.