Oral Administration of Euterpe oleracea Mart., Its Influence over Body Weight and Lymphoid Organs and Alterations in Haematological and Glycaemical Parameters.
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Date
2014-11
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Abstract
Aims: The aims of the study were to evaluate alterations caused by the oral
administration of the aqueous extract of Euterpe oleracea to non-isogenic adult male mice
during 15 and 30 consecutive days through the comparison of body and spleen weights,
number of splenic and medullary cells, their hemogram and glycemia indices.
Study Design: Animals were divided into 8 groups (5 animals/group). Treated groups
received the AEA concentration (125 mg/ml by gavage) at doses of 5, 50 and 500 mg/kg
for 15 and 30 days. The control group received only the vehicle of dilution (1x PBS) in
volume of 0.5 ml. After treatment the animals were sacrificed in a CO2 chamber for
testing.
Place and Duration of Study/Methodology: The aqueous extract of concentration of
125 mg/ml of lyophilized açaí pulp was prepared in Laboratory of General and Analytical
Chemistry of Federal University of Amapá. Values of body weight, weight of liver and
spleen, number of spleen and bone marrow cells, blood count and glucose of the mice
were conducted on Drugs Research Laboratory between January and November of 2013.
Results: The statistical analysis was done by ANOVA test two-way with significance level
p<0.05 in relation to control followed by Tukey post-test. The AEA caused significant
changes in body weight, about 22% in animals treated with 500mg/kg. Weight of spleen
showed no significant changes, there was statistical difference in blood glucose levels
between groups 5 and 500 mg/kg treated for 15 days and punctually in the 500 mg/kg
group treated for 15 and 30 days. It was observed that the treatment with the AEA doses
(5, 50 and 500 mg/kg) for 30 days increased the number of bone marrow cells. Regarding
the number of spleen cells, treatment promoted changes, reducing the amount of cells
during the 30 days of treatment, principally at the dose of 50 mg/kg. During the 15 day
treatment of 500 mg/kg there was an increase in the number of spleen cells.
Conclusion: The treatment of mice with aqueous extract of açaí pointed that the
concentration has significant influence in: 1) glucose concentration in the blood; 2) The
increased number of bone marrow cells; 3) and the number of spleen cells. Thus,
comparison between groups of animals was compatible with the hypothesis that the
increase in body mass is a risk factor for insulin resistance.
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Keywords
Açai, Euterpe oleracea mart, spleen, mice
Citation
Pinheiro Mayara T, Silva Nayara S R, Lobato Cleison C, Braga Francinaldo S, Santos César F, Costa Josivan S, Carvalho José Carlos T, Santos Cleydson Breno R. Oral Administration of Euterpe oleracea Mart., Its Influence over Body Weight and Lymphoid Organs and Alterations in Haematological and Glycaemical Parameters. British Journal of Pharmaceutical Research. 2014 Nov; 4(21): 2484-2493.