Biosynthesis of Silver Nanoparticles Using Callus Extract of Catharanthus roseus var. alba and Assessment of Its Antimicrobial Activity.
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Date
2014-07
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Abstract
Objective: To express a cost efficacious and environment friendly method for the green
synthesis of silver nanoparticles (AgNPs) from Catharanthus roseus var. alba (C. roseus
var. alba) callus extract.
Methodology: The aqueous solution of sliver nitrate containing Ag+ ions (1 mM) are
integrating 100 μL of aqueous extract of callus of C. roseus var. alba and 10 mL of 1%
w/v aqueous solution of polyethylene glycol 4000 to 90 mL. This was then alkalized with
0.1 NaOH (20 μL) and treated in a microwave oven (800 W) for 40 sec for the reduction of
metal ion and the reaction takes place at room temperature (250C). The reduction of the
Ag+ ions by aqueous callus extract of C. roseus var. alba in the solutions was monitored
by UV–Visible spectrum and further characterized by dynamic light scattering (DLS),
transmission electron microscopy (TEM) and x-ray diffraction (XRD). Antibacterial activity
was assessed on bacterial strain of Staphylococcus aureus and Pseudomonas
aeruginosa (S. aureus and P. aeruginosa) by using the disc-diffusion assay method.
Results: Characterization of AgNPs was done DLS, TEM and XRD methods. The
Dynamic Light Scattering (DLS) showed the particle size distribution of the AgNPs synthesized from C. roseus var. alba callus extract was found 86.95 nm with
polydispersity index (PDI) value of = 0.304. TEM images showed the formation of AgNPs
with an average size of 10 nm to 20 nm. And the XRD analysis showed that the AgNPs
were crystalline in nature with face-centered-cubic (FCC). For the assessment of
antibacterial activity the concentration of AgNPs 25 μL, 50 μL, 100 μL and 150 μL were
used against both the bacterial strain S. aureus and P. aeruginosa, the zone of inhibition
found 4 mm, 7 mm, 16 mm and 23 mm as well as 5 mm, 9 mm, 19 mm and 26 mm
respectively.
Conclusions: Aseptically engendered callus of C. roseus var. alba demonstrates
vigorous potential for synthesis of silver nanoparticles by rapid reduction of Ag+ to Ag.
The biologically synthesized AgNPs showed more preponderant antibacterial effect
against S. aureus and P. aeruginosa.
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Keywords
AgNPs, Catharanthus roseus var. alba, UV-Vis spectrophotometry, DLS, TEM, XRD
Citation
Barkat Md Abul, Mujeeb Mohd, Samim Md, Verma Shashikant. Biosynthesis of Silver Nanoparticles Using Callus Extract of Catharanthus roseus var. alba and Assessment of Its Antimicrobial Activity. British Journal of Pharmaceutical Research. 2014 July; 4(13): 1591-1603.