Optimization of the Cultural Parameters for Improved Production of Antimicrobial Metabolites by Streptomyces gulbargensis DAS 131.
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Date
2014-05
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Abstract
Aims: To investigate the influence of appropriate culture medium by optimizing the
cultural conditions affecting the growth and bioactive metabolite production by
Streptomyces gulbargensis DAS 131 under submerged culture conditions in order to
reduce the cost of fermentation process to improve the formation of antimicrobial
compounds.
Place and Duration of Study: Department of Botany and Microbiology, January 2012 to
May 2012.
Methodology: The impact of environmental parameters such as incubation period, pH,
temperature and salt concentration and effect of various nutrients such as carbon and
nitrogen sources and minerals on the antimicrobial metabolite production by
Streptomyces gulbargensis DAS 131 was evaluated by employing agar well diffusion assay. Growth was measured in the form of dry mycelial weight.
Results: The optimum pH and temperature for bioactive metabolite production were 7
and 35°C respectively. Highest antimicrobial metabolite production was found when the
strain was inoculated into the medium amended with glucose at the concentration of 2%,
soya peptone at the rate of 1% and NaCl at the concentration of 5% and incubated for six
days under shaking conditions. The metabolites showed good antimicrobial activity
against Gram positive and Gram negative bacteria, as well as unicellular and multicellular
fungi.
Conclusion: S. gulbargensis DAS 131 isolated from the semi-arid soils of Gulbarga,
Northern Karnataka province, India exhibited broad spectrum antimicrobial activity. It was
found that the antimicrobial metabolite production by the strain was positively influenced
by carbohydrates, nitrogen sources and minerals.
Description
Keywords
Optimization, bioactive metabolites, nutritional factors, antimicrobial activity, Streptomyces gulbargensis DAS 131
Citation
Mangamuri Usha Kiranmayi, Vijayalakshmi Muvva, Poda Sudhakar, Agasar Dayanand. Optimization of the Cultural Parameters for Improved Production of Antimicrobial Metabolites by Streptomyces gulbargensis DAS 131. British Journal of Pharmaceutical Research. 2014 May; 4(9): 1130-1145.