Wound Healing Activity of Premna latifolia Roxb.
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Date
2014-04
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Abstract
Aims: The study aims to understand the wound healing potentials of a 50% aqueous
ethanolic extract of Premna latifolia stem using excision wound model.
Study Design: The wound healing potentials were simultaneously supported by
observing the bacterial functional diversity of wound swabs using Biolog Eco plates. The
antioxidant activity was performed using In vitro DPPH free radical scavenging assay.
Place and Duration of Study: CSIR-National Botanical Research Institute (NBRI),
Lucknow, between May 2013 and November 2013.
Methodology: Wound healing activity of the plant was studied using excision wound
model. Animals were divided into three groups of six male rats each as control group (GI)
dressed with compound free simple ointment. Test group (GII) treated with 50% aqueous
ethanolic extract of P. latifolia stem (10% w/w) in ointment vehicle and standard group
(GIII) group treated Nitrofurazone ointment, Himedia (0.2%w/w). The wound healing
potential was further supported by the DPPH free radical scavenging and antibacterial
activity of the plant. The phytochemical estimations were done using standard methods.
Results: Sugar and starch content in the plant was 3.55% and 5.54% respectively. Total
tannins, phenol and flavonoid content were estimated to be 0.18%, 0.54% and 2.73%. The 50% ethanolic extract of the plant showed moderate DPPH free radical scavenging
activity with an IC50 of 188.02μg/ml. A 69.15% of wound closure was observed on 10th day
post wounding of the rats treated with 200 mg/kg of extract. The results also indicated
significant antibacterial activity of the extract.
Conclusion: The 50% aqueous ethanolic extract of P. latifolia shows significant wound
healing activity.
Description
Keywords
Premna latifolia, free radical, antibacterial, phytochemical, wound healing
Citation
Pandey Garima, Chaudhary V, Yadav S, Chauhan P S, Gupta Shyam Sundar, Mishra A, Rawat A K S, Rao Ch V. Wound Healing Activity of Premna latifolia Roxb. British Journal of Pharmaceutical Research. 2014 Apr; 4(8): 929-942.