Quantitative Estimation of Piperine and Diosgenin in Ayurvedic Formulation.
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Date
2014-04
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Abstract
Goksuradi guggulu is a polyherbal formulation official in “Ayurvedic formulary of India” and
used for dysuria, urinary obstruction, increased frequency and turbidity of urine, calculus,
excessive vaginal discharge, gout. A simple, specific and precise high performance thinlayer
chromatography (HPTLC) method has been developed for quantification of piperine
and diosgenin in Goksuradi guggulu. We report the extraction and estimation of these
compounds in a laboratory prepared sample of Goksuradi guggulu and two of its
marketed formulations. The compounds were chromatographed on precoated silica gel G
60 254 plates in the mobile phase comprising of toluene: hexane: ethyl acetate
(6.8:0.2:3). Under the optimized chromatographic conditions, the calibration plot was
found to be linear in the range of 0.2-1μg spot-1 for piperine and 1.0 -3.0 μg spot-1 for
diosgenin. The correlation coefficient (R2) was 0.9979 and 0.9915 for piperine and
diosgenin respectively. Mean recovery (% w/w) for piperine in all the formulations was in
the range of 97.61 - 98.90 and for diosgenin, it was 93.76 - 94.33.
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Keywords
Piperine, diosgenin, HPTLC, quantitation
Citation
Parameswaran Sandhya, Koshti Shalaka. Quantitative Estimation of Piperine and Diosgenin in Ayurvedic Formulation. British Journal of Pharmaceutical Research. 2014 Apr; 4(8): 910-919.