Analgesic, Anti-inflammatory and CNS Depressant Activities of the Methanolic Extract of Abelmoschus esculentus Linn. Seed in Mice.
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Date
2014-04
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Abstract
Aims: The study was carried out to assess the analgesic, anti-inflammatory and CNS
depressant activity of the methanolic extract of Abelmoschus esculentus Linn. Seeds.
Study Design: The Present study was designed to observe pharmacological activities of
the crude extract of the plant Abelmoschus esculentus seeds. The study consisted of hot
extraction of the seeds of the A. esculantus with methanol. Afterwards, Methanolic crude
extract was filtered and the filtrate was evaporated. Finally, screening of analgesic, antiinflammatory
and CNS depressant activity of crude extracts of A. esculantus on Swiss
Albino mice.
Place and Duration of Study: Department of Pharmacy, Atish Dipankar University of
Science and Technology, Dhaka, Bangladesh. January, 2013- July 2013.
Methodology: The animals are divided into Four groups and each group consists of five mice. Analgesic activity was performed by acetic acid-induced writhing model and
formalin induced licking and biting in mice. Anti-inflammatory effects of Abelmoschus
esculentus seed extract were done by carrageenan induced anti-inflammatory method at
the dose of 100 and 200 mg/kg b.wt., (p.o). The CNS depressant activity was evaluated
by observing the reduction of locomotor and exploratory activities in the hole cross and
opens field tests at the dose of 100 and 200 mg/kg body weight.
Results: In statistical analysis, the dose (200 mg/kg) was found to exhibit (significant
p=0.05) better analgesic activity (65.16% and 54.38%) against both acetic acid and
formalin induced pain in mice which is about similar to standard drug Indomethacin. The
extract of A. esculentus (100 and 200mg/kg) also showed sustained inhibition (54.97%
and 65.56%) of paw edema at the 4th hour compared to Indomethacin (74.17%). Besides
this A. esculentus (significant p=0.05) seed extract (100 and 200mg/kg p.o.) also
possesses depressant activity at 90min in both methods. Conclusion: this study
recommends that the methanolic extract of the Abelmoschus esculentus seeds has
significant CNS depressant, analgesic and anti-inflammatory properties.
Description
Keywords
Abelmoschus esculentus, analgesic, Formalin induced pain, anti-inflammatory, CNS Depressant
Citation
Nesa Mst Luthfun, Islam Md Monirul, Alam Md Badrul, Munira Mst Shirajum, Mollika Shabnam, Naher Mst Nazmun, Islam Md Rafiqul. Analgesic, Anti-inflammatory and CNS Depressant Activities of the Methanolic Extract of Abelmoschus esculentus Linn. Seed in Mice. British Journal of Pharmaceutical Research. 2014 Apr; 4(7): 849-860.