Angiogenesis and Antioxidant Activity of in vitro and in vivo Tuber of Ceropegia pusilla Wight and Arn.
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Date
2014-03
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Abstract
Background: Angiogenesis refers to the formation of new blood vessels. This biological
process lays an important role in embryonic development. Ceropegia pusilla
(Asclepiadaceae) used in folk medicine system to treat various health problems. Present
work deals with the analysis of antioxidant and antiangiogenic properties of in vivo and in
vitro tuber extracts of C. pusilla by Chicken Egg Chorioallantoic Membrane Assay (CAM)
in vivo.
Methods: C. pusilla tubers of both in vivo and in vitro were extracted with ethanol and
methanol. In vivo anti angiogenesis effect was studied by Chicken Egg Chorioallantoic
Membrane (CAM) assay. The antioxidant properties were analysed using 1, -diphenyl-2-
picryl- hydrazyl free radical scavenging assay and reducing power activity.
Result: Ethanolic extract of both in vivo and in vitro tuber showed higher angiogenic
activities 58.3±13.6 and 43.2±12.2 respectively than the methanolic extract. The average
number of vessels in in vivo and in vitro ethanolic extracts treated CAM was 6.48 and
5.43. In vivo and in vitro extracts exhibits comparable DPPH radical scavenging activity and better reducing power activity.
Conclusion: C. pusilla tuber extracts of both in vivo and in vitro inhibited angiogenesis by
blocking normal vascularisation in chick embryo and also having better DPPH and
reducing power activity.
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Keywords
Angiogenesis, antioxidant, Ceropegia pusilla, reducing power
Citation
Prabakaran R, Kalimuthu K, Vani C, Brindha C. Angiogenesis and Antioxidant Activity of in vitro and in vivo Tuber of Ceropegia pusilla Wight and Arn. British Journal of Pharmaceutical Research. 2014 Mar; 4(5): 608-616.