Formulation and Characterization of Nystatinloaded Nanostructured Lipid Carriers for Topical Delivery against Cutaneous Candidiasis.
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Date
2014-02
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Abstract
Aims: The objective of the current study was to formulate nystatin (Nyst) into
nanostructured lipid carriers (NLCs) to enhance its antifungal activity.
Place and Duration of Study: Department of pharmaceutical technology, national
research centre, Egypt, between mars 2011 to april 2013
Methodology: Nyst loaded NLCs (NYST-NLCs) were prepared by the hot
homogenization and ultrasonication method followed by evaluation of its topical effect on the cutaneous candidiasis. The prepared Nyst-NLCs were characterized for entrapment
efficiency, particle size, zeta potential, morphology (transmission electron microscopy),
thermal characterisation (differential scanning calorimetry) and in vitro drug release. The
study design involves the investigation of the effect of three independent variables namely
liquid lipid type (Miglyol 812 and Squalene), liquid lipid concentration (30 and 50%) and
Nyst concentration (0.125 and 0.25%). A stability study for 6 months was performed. A
microbiological study was conducted in male rats infected with Candida albicans.
Results: NLC dispersions were spherical in shape with particle size ranging from
68.06±6.56 to 141.8±3.33 nm. The entrapment efficiencies ranged from 45.50±2.34 to
92.73±0.33% with zeta potential ranging from -26.2 to -39.2 mV. The stability studies
done for 6 months indicated that Nyst-NLCs were stable for more than 6 monthes.the
microbiological studies showed A least number of colonies forming units (cfu/ml) were
recorded for the selected Nyst-NLCs compared to the drug solution and the Nystatin®
cream present in the market.
Conclusion: It can be fulfilled from this work that NLCs represent promising carrier for
topical delivery of Nyst offering good physical stability, high entrapment efficiency and
controlled drug release. Nyst-NLCs are a good candidate for cutaneous treatment of
fungal infection.
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Keywords
Nanostructured lipid carriers, characterization, Nystatin, fungal infection, Candida albicans
Citation
Khalil Rawia M, Abd- Elbary A, Kassem Mahfouz A, El Ridy Mohamed S, Abou Samra Mona M, Awad Ghada E A, Mansy Soheir S. Formulation and Characterization of Nystatinloaded Nanostructured Lipid Carriers for Topical Delivery against Cutaneous Candidiasis. British Journal of Pharmaceutical Research. 2014 Feb; 4(4): 490-512.