Exploration of the Angiogenic Potential of Honey.
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Date
2014-02
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Abstract
Aims: The present study was conducted to confirm the angiogenic potential of honey
using Chick Chorioallantoic Membrane (CAM), an in ovo model and to study its effect on
Vascular Endothelial Growth Factor (VEGF) expression in the CAM tissue. Attempts were
also made to identify the probable active constituents present in honey that contributed to
its angiogenic potential.
Methodology: Honey was evaluated over concentrations ranging from 0.015 to 25% v/v
and the extent of angiogenesis was quantified using stereomicroscopy. VEGF expression
at transcript level was determined by RT-PCR. Erythropoietin and Heparin were used as
positive and negative controls respectively. Four known constituents of honey viz.,
Glucose, Proline, Vitamin C and Hydrogen peroxide were tested by biochemical methods.
Results: New blood formation was seen at all the concentrations tested, however the proangiogenic
effect was greater at lower concentrations. These results were significantly
greater than that seen with erythropoietin, the positive control. VEGF mRNA expression in
CAM tissue also demonstrated similar findings. Among the constituents tested, Vitamin C
and Hydrogen peroxide were observed to be associated with the angiogenic effect of
honey.
Conclusion: The study thus confirms the pro-angiogenic potential of honey at low concentrations. This effect is probably due to the presence of Hydrogen peroxide and
Vitamin C and is mediated via alteration in VEGF expression.
Description
Keywords
Honey, angiogenesis, VEGF, Chorioallantoic membrane, glucose, proline, hydrogen peroxide, stereomicroscopy
Citation
Munshi R M, Bhalerao S S, Kalekar S A, Patil T A. Exploration of the Angiogenic Potential of Honey. British Journal of Pharmaceutical Research. 2014 Feb; 4(4): 477-489.