In vitro Antioxidant, Antihyperglycaemic and Antihyperlipidaemic Activities of Ethanol Extract of Lawsonia inermis Leaves.
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Date
2014-02
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Abstract
Aim: This present study investigated the free radical scavenging activities,
antihperglycaemic and antihyperlipidaemic activities of ethanol extract of Lawsonia
inermis leaves.
Study Design: Twenty male rats were randomly and evenly distributed into four groups,
and were subsequently exposed to the following treatments for twenty-one days: Group I
(Control): Normal saline; Group II: Untreated Diabetic control; Group III: Diabetic rats
treated with glibenclamide (600mg/Kg. b.wt); Group IV: Diabetic rats treated with ethanol
extract of Lawsonia inermis (400mg/Kg b.wt).
Place and Duration of Study: This work was carried out in the Department of
Biochemistry, University of Lagos, Lagos, Nigeria between November 2012 and February
2013.
Methodology: Phytochemical screening and the antioxidant activities of the plant extract were carried out. Brine shrimp lethality assay was also carried out on the plant extract for
In vitro toxicity assay. Blood samples collected from the experimental rats were used for
the determination of fasting blood sugar and biochemical profiles following the last oral
treatment and an overnight fast.
Results: Lawsonia inermis showed maximum inhibition of 77.39%, 50.78% and 83.18 %
at 100μg/ml DPPH free radical scavenging activity, in vitro lipid peroxidation and nitric
oxide respectively. Following twenty-one days of treatment with oral administration of
ethanol extract of Lawsonia inermis leaves, fasting blood glucose was significantly
reduced (P<0.001) compared to the untreated diabetic control. Activities of AST, ALT, and
ALP were significantly decreased (P<0.001) in the diabetic rats treated with the extract
compared to the untreated diabetic control rats. Diabetic rats treated with ethanolic leaf
extract of Lawsonia inermis showed statistically significant decrease (P<0.001) in the
levels of total cholesterol, total triglyceride and low density lipoprotein cholesterol when
compared with untreated diabetic rats.
Conclusion: The ethanol extract of Lawsonia inermis leaves showed antioxidant,
antihyperglycaemic and antihyperlipidaemic activities.
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Keywords
Brine shrimp lethality assay, free radical scavenging activities, antihyperglycaemic activity, Lawsonia inermis
Citation
Ojewunmi Oyesola O, Oshodi Tope, Ogundele Omobolanle I, Micah Chijioke, Adenekan Sunday. In vitro Antioxidant, Antihyperglycaemic and Antihyperlipidaemic Activities of Ethanol Extract of Lawsonia inermis Leaves. British Journal of Pharmaceutical Research. 2014 Feb; 4(3): 301-314.