In vitro Antioxidant, Cytotoxic and Antibacterial Screening of the leaves of Acridocarpous orientalis, Native to Sultanate of Oman.
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Date
2013-10
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Abstract
Aims: To determine antioxidant, cytotoxic and antimicrobial activities of organic extracts
from leaves of Acridocarpous orientalis (qafas) from Sultanate of Oman.
Study Design: Brine shrimp test, DPPH assay and Disc diffusion method.
Place and Duration of Study: School of Pharmacy, University of Nizwa, Oman,
December 2012.
Methodology: Hexane, chloroform, ethylacetate and hydroalcoholic extract were
obtained by Kupchan’s partitioning of ethanol extract isolated from leaves of A. orientalis
by maceration. Antioxidant activity was determined by free radical scavenging of (2,2-
diphenyl-1-picrylhydrazyl, DPPH). The antimicrobial activity was checked using agar disc
diffusion method against Gram-positive bacteria (Staphylococcus aureus) and two Gramnegative
bacteria (Escherichia coli and Pseudomonus aeruginosa). Brine shrimp test
was used to measure cytotoxic activity.
Results: All extracts demonstrated potential antioxidant activities, hydroalcoholic extract
showed the strongest activity (RC50 = 6.11 g/ml). The order of antioxidant activity was
hydro alcohol > ethyl acetate > chloroform > hexane extract. Ethylacetate extract showed
low activity against Pseudomonus aeruginosa. None of the extracts was found to be active against brine shrimp larvae.
Conclusion: A. orientalis could be considered as a good source of antioxidant
compounds.
Description
Keywords
Aridocarpous orientialis, qafas, DPPH, Oman, antimicrobial, antioxidant, cytotoxic
Citation
Al-Abri Ahlam, Al-Maqbali Hazaa, Weli Afaf, Said Sadri, Hossain Amzad, Akhtar Md Sohail. In vitro Antioxidant, Cytotoxic and Antibacterial Screening of the leaves of Acridocarpous orientalis, Native to Sultanate of Oman. British Journal of Pharmaceutical Research. 2013 Oct; 3(4): 734-742.