Hepatoprotective Effect of Drynaria quercifolia Fronds Hydroalcoholic Extract and Isolated Constituent against CCl4-Induced Hepatocellular Damage.
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Date
2013-10
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Abstract
Aims: The present study was conducted to evaluate the hepatoprotective effect of
hydroalcoholic extract of Drynaria quercifolia fronds (Dq), its fractions and isolated
compound (Dq-4) from ethyl acetate (EA) fraction.
Place and Duration of Study: Department of Pharmacognosy and Department of Herbal
Drug Research, ISF College of Pharmacy, Moga, between June 2010 and May 2012.
Methodology: The toxicant CCl4 (1ml/kg) was administered on 4th and 5th day to induce
hepatotoxicity in Wistar rats (in-vivo) and the in-vitro hepatoprotection was evaluated
against CCl4 (1%) induced toxicity in HepG2 cellines.
Results: The pre-treatment of rats with Dq extract, EA fraction and Dq-4 for 7 days
produced a significant dose dependent hepatoprotective action by decreased levels of
hepatic enzymes, total bilirubin and TBARS and increased levels of total proteins,
albumin, and reduced glutathione. The histological examination provided the supportive
evidences. Additionally, Dq extract, EA fraction and Dq-4 significantly decreased the CCl4-induced in-vitro toxicity in HepG2 cellines evident by MTT reduction assay and
trypan blue method.
Conclusion: The study scientifically validated the traditional use of D. quercifolia for liver
disorders and strongly demonstrates antioxidative effect on hepatocytes in restoring their
normal architecture and functional ability.
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Keywords
Hepatoprotective, HepG2, Drynaria quercifolia, Naringin
Citation
Kamboj Pradeep, Kalia Ajudhia Nath. Hepatoprotective Effect of Drynaria quercifolia Fronds Hydroalcoholic Extract and Isolated Constituent against CCl4-Induced Hepatocellular Damage. British Journal of Pharmaceutical Research. 2013 Oct; 3(4): 563-578.