Potential of Probiotic Lactobacillus plantarum 2621 for the Management of Infertility.
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Date
2014-12
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Abstract
Background: Infertility outcomes may be associated with the infections that would lead to
morphological defects of spermatozoa in vitro. The purpose of this work was to investigate
the effect of Lactobacillus plantarum 2621 (L. plantarum) on adherence of sperm
agglutinating Escherichia coli (E. coli) in vitro and in vivo as well as its effect on fertility
outcome.
Materials and Methods: Interference of E. coli adherence to vaginal epithelial cells
(VECs) by L. plantarum was studied by carrying out different assays such as exclusion,
competition and displacement. Further, in vivo study was carried out in mouse model to evaluate the effect of presence of L. plantarum against E. coli and its effect on fertility
outcome by administering intravaginally at one hour interval between L. plantarum
(108c.f.u./20μl) and different concentrations of E. coli (102,104, and 106 c.f.u/20 μl) for ten
consecutive days.
Results: 116•8 bacteria/VEC adhesion levels were observed for L. plantarum 2621
whereas values for E. coli were 60•5 bacteria/VEC. L. plantarum interfered to different
extents with the adherence of E. coli. L. plantarum 2621 decreased the adhesion by
displacement and competition in a significant level (90.3% and 68.5% of inhibition).
L. plantarum 2621 also excluded the E. coli attached to VEC (25.8% of inhibition).Upon
mating and completion of gestation period 100% fertility was observed with 108c.f.u./20μl
L. plantarum and 102 c.f.u/20μl E. coli, whereas 100% females were infertile when
administered with 106 c.f.u/20μl of E. coli alongwith 108c.f.u./20μl L. plantarum and only
50% fertility outcome was observed with 104 c.f.u/20μl E. coli.
Conclusion: Results indicated that L. plantarum displaces colonization of E. coli and
endows competition that resulted in reinforcement of natural microflora and affects fertility
outcome depending on the presence and count of E. coli.
Description
Keywords
Adherence, Infertility, Lactobacillus plantarum, Escherichia coli, Intravaginal administration
Citation
Bhandari Praveen, Rishi Praveen, Prabha Vijay. Potential of Probiotic Lactobacillus plantarum 2621 for the Management of Infertility. British Microbiology Research Journal. 2014 Dec; 4(12): 1585-1596.