Partial Purification and Antimycobacterial Screening of the Ethyl Acetate Extract of Alcaligenes faecalis BW1.
Loading...
Date
2014-11
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aims: The focus of this study was to evaluate the antimycobacterial activity of Alcaligenes
faecalis BW1 extract and to purify it partially.
Study design: Partial purification of A. faecalis BW1 extract was performed by using thin
layer chromatography and active substances responsible for the biological activity were
localized.
Place and Duration of Study: The study was carried out at laboratory of Microbial
Biotechnology, Department of Biology, Faculty of Sciences and Technical, University Sidi
Mohamed Ben Abdellah, BP 2202, Road of Immouzer, Fez, Morocco, during the period
from January 2011 to July 2011.
Methodology: Crude extract of A. faecalis BW1 was obtained by using ethyl acetate as an
organic solvent and its antimycobacterial effect was investigated by agar discs diffusion
method. The extract was then fractionated by thin layer chromatography and the bioactivity
was assessed with a bioautography technique followed by spots elution tests.
Results: The results showed that A. faecalis BW1 produced compounds with
antimycobacterial activity. All the detected spots by thin layer chromatography inhibited the
growth of M. smegamtis. Conclusion: Various metabolites of A. faecalis BW1 are responsible for the sought effect
or they could act synergistically to inhibit mycobacterial growth. These compounds could be
used after their total purification in further work against mycobacterial infections.
Description
Keywords
Alcaligenes faecalis, BW1 extract, thin layer chromatography, bioautography
Citation
Zahir Ilham, Houari Abdellah, Iraqui Mohammed, Ibnsouda Saad. Partial Purification and Antimycobacterial Screening of the Ethyl Acetate Extract of Alcaligenes faecalis BW1. British Microbiology Research Journal. 2014 Nov; 4(11): 1178-1188.