Electrostatic and Water Content Effects on Yarrowia lipolytica Lipase Immobilization by Adsorption.
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Date
2014-06
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Abstract
Aims: In the present investigation, an attempt has been made to explain lipase
immobilization by adsorption on three minerals matrixes, i.e. Celite 545, Silica gel (60G)
and Avicel (PH 101).
Study Design: immobilization by absorption on minerals matrixes, water content by
volumetric karl Fischer titration and surface potentials using a particle charge detector
Mutek PCD 03 were used.
Place and Duration of Study: Walloon Centre of Industrial Biology (CWBI) Unit of Bio-
Industries, University of Liege, Gembloux Agro-Bio Tech, Passage des Deportes 2, B-
5030 Gembloux, Belgium between Jun 2012 and jun 2013.
Methodology: A methodical order was developed whereby the influences of water content, surface potentials and pH, on immobilization by adsorption were explored.
Adsorbed YLL was used to understand an interesterification reaction between rapeseed oil
and milk fat in comparison with a commercial silica-granulated Thermomyces lanuginosus
lipase (Lipozyme TL IM).
Results: Maximum immobilization yield was obtained with Celite (70%) and the lowest
with silica gel (29%). Total water content of free and immobilized lipase was determined by
volumetric Karl Fischer titration. The water content of Silica gel was higher than the one of
other supports. Water content of silica gel could prevent the enzyme fixation. These
results could be explained by the adsorption being governed mainly by electrostatic
interactions between the enzyme and matrix. This hypothesis was further reinforced by
measurements of electrical potential. They showed a lowest negative potential of Silica gel
after enzyme adsorption in comparison to Celite.
Conclusion: From these results celite was designated as an efficient matrix to immobilize
Yarrowia lipolytica lipase (YLL) by adsorption. This performed system was used to realize
an interesterification reaction between rapeseed oil and milk fat in comparison with a
commercial silica-granulated Thermomyces lanuginosus lipase (Lipozyme TL IM).
Description
Keywords
Yarrowia lipolytica lipase, celite, silica gel, avicel, immobilization, water content, electrostatic interaction, interesterification
Citation
Alloue-Boraud Wazé A Mireille, Lejeune Annick, Koffi-Nevry Rose, Destain Jacqueline, Paquot Michel, Thonart Philippe. Electrostatic and Water Content Effects on Yarrowia lipolytica Lipase Immobilization by Adsorption. British Microbiology Research Journal. 2014 June; 4(6): 640-653.