Enzymatic Activity of Lactobacillus Grown in a Sweet Potato Base Medium.
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Date
2014-05
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Abstract
Aim: To study the impact of sweet potatoes on α-glucosidase, β-glucosidase, acid
phosphatase, and phytase activity of Lactobacillus.
Study Design: Enzymatic activity of seven strains of Lactobacillus grown in a sweet potato
medium (SPM) was determined and compared to the standard lactobacilli MRS. Strains
having the highest enzymatic activity were further enhanced by metal ions.
Place and Duration of Study: Food Microbiology and Biotechnology Laboratory, North
Carolina A&T State University, Greensboro, NC, USA, between September 2012 and May
2013.
Methodology: Lactobacillus strains were grown in SPM and MRS at 37ºC for 16 h. At the
end of incubation, bacterial population (log CFU/mL) was determined by plating and
enzymatic activity was determined spectrophotometrically using the corresponding
substrate.
Results: Lactobacillus strains continue to grow in SPM and MRS and reached averages of
10.98±0.49 and 10.92±0.55 log CFU/mL respectively. Growth of Lactobacillus strains in
SPM led to higher β-glucosidase, acid phosphatase, and phytase activity than MRS. Strains
of L. reuteri (CF2-7F and SD2112) grown in SPM showed the highest acid phosphatase
(15.84±1.05 and 20.56±1.49 Ph U/mL), and phytase (0.66±0.14 and 0.65±0.11 Ph U/mL)
respectively. The highest β-glucosidase (36.04±3.16 Glu U/mL) activity was obtained from L. delbrueckii subsp. bulgaricus SR35 grown in SPM. In addition, acid phosphatase and
phytase produced by L. reuteri CF2-7F growing in SPM were further increased by the
addition of Mn2+ (70.1 and 41.8%) or Mg2+ (94.7 and 20.9%) respectively. β-glucosidase
activity of L. reuteri was increased in a range of 4.1 to 130.6% due to the addition of metal
ions.
Conclusion: Components in sweet potatoes could increase enzymatic activity of
Lactobacillus and the addition of metal ions could further produce an enhanced level of
these enzymes.
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Keywords
Sweet potato medium, Lactobacillus, enzymatic activity, metal ions and L. reuteri
Citation
Hayek Saeed A, Shahbazi Aboghasem, Worku Mulumebet, Ibrahim Salam A. Enzymatic Activity of Lactobacillus Grown in a Sweet Potato Base Medium. British Microbiology Research Journal. 2014 May; 4(5): 509-522.