Behavioral Patterns and Expression of Genes Coding Serotonin Receptors in Rats with Ultrasound Induced Depression.
Loading...
Date
2013-10
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aims: The aim of our study was to investigate the effects of continuous action of
ultrasonic waves of variable frequencies on behavior of rats in "classical" tests used to
reveal depression-like behavior, to evaluate the influence of different psychotropic drugs
on rates of these tests and to analyze expression of several genes involved in
pathogenesis of depression.
Study Design: Rats in individual cages were exposed to ultrasonic irradiation for 21
days.
Place and Duration of Study: V.P. Serbsky National Recearh Center For Social and
Forensic Psychiatry, Department of Basic and Applied Neurobiology, Moscow, Russian Federation, between November 2012 and January 2013.
Methodology: 48male non-pedigree albino rats were divided into 5 groups: nonultrasound-
saline, ultrasound-saline, ultrasound-fluoxetine, ultrasound-bupropion and
ultrasound-tianeptine. After 21 days of irradiation social interaction test, forced swimming
test and sucrose preference test (anhedonia test) were conducted. Than rats were
decapitated and prefrontal cortex were taken for RT-qPCR gene expression analysis of
5-HT1A, 5-HT2A, 5HT1B, 5HT2B receptors and SERT.
Results: Depression-like behavior manifests itself in reduced social activity in social
interaction test, increased immobility in forced swimming test and lower sucrose
consumption in anhedonia test. The administrated antidepressants demonstrated their
effectiveness, except for bupropion in the social interaction test. RT-qPCR gene
expression analysis showed reduced expression of 5HT2A receptor gene and increased
expression of SERT gene in the prefrontal cortex of rats stressed with ultrasonic
radiation.
Conclusion: The obtained data allow to conclude that further investigations with lager
number of animals, extended tests battery may allow to claim that this model meets the
main requirements set to animal models (face, predictive and construct validity) and can
be used in studies of depression-like disorders caused by a situation of informational
uncertainty and in pre-clinical development of new antidepressants.
Description
Keywords
Ultrasonic radiation, animal model, 5-HT2A receptor, SERT, behavior, antidepressants, rats
Citation
Morozova A Y, Zubkov E A, Storozheva Z I, Koshkin P A, Shepeleva I I, Kekelidze Z I, Chekhonin V P. Behavioral Patterns and Expression of Genes Coding Serotonin Receptors in Rats with Ultrasound Induced Depression. British Journal of Medicine and Medical Research. 2013 Oct-Dec; 3(4): 2107-2118.