Optimization of Process Parameters for Improved Production of Bioactive Metabolites by Streptomyces tritolerans DAS 165T.
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Date
2014-04
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Abstract
Aims: To optimize the process parameters for enhanced production of bioactive
metabolites by Streptomyces tritolerans DAS 165T.
Place and Duration of Study: Department of Botany and Microbiology, April 2012 to
August 2012.
Methodology: Agar well diffusion assay was employed to study the effect of environmental
parameters such as incubation period, pH, temperature and salt concentration and
influence of various nutrients such as carbon and nitrogen sources and minerals on the
bioactive metabolite production by Streptomyces tritolerans DAS 165T.
Results: The production of antimicrobial metabolite was high when the strain was cultured
for six days at 35ºC in medium (pH 7.5) with sucrose at the concentration of 2% (carbon
source), soya peptone at the concentration of 1% (nitrogen source) and sodium chloride at
the concentration of 5%.
Conclusion: This is the first report on the optimization of bioactive metabolite production by Streptomyces tritolerans DAS 165T. As the strain exhibited potent antimicrobial activity, it
may be explored for biotechnological purposes.
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Keywords
Optimization studies, streptomyces tritolerans, bioactive metabolites, environmental parameters, nutritional factors, antimicrobial activity
Citation
Mangamuri Usha Kiranmayi, Muvva Vijayalakshmi, Poda Sudhakar, Agasar Dayanand. Optimization of Process Parameters for Improved Production of Bioactive Metabolites by Streptomyces tritolerans DAS 165T. British Microbiology Research Journal. 2014 Apr; 4(4): 428-442.