Phenotypic and Genotypic Characteristics of Rhizobia Isolated from Meknes-tafilalet Soils and Study of Their Ability to Nodulate Bituminaria bituminosa.
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Date
2014-04
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Abstract
Aims: The objectives were to isolate and characterize phenotypically and genotypically the
rhizobial strains from the soils belonging to the Meknes-Tafilalet region in order to select
strains that are able to nodulate Bituminaria bituminosa.
Study Design: An experimental study.
Place and Duration of Study: Department of biology (Soil & Environment Microbiology
Unit) Faculty of Sciences, Moulay Ismail University and Technical Support Unit for Scientific
Research, CNRST in Rabat; between January and August 2010.
Methodology: Samples from 23 different sites belonging to the Meknes-Tafilalet region
were collected in order to select rhizobial strains that are able to nodulate Bituminaria
bituminosa. The morphological, cultural and phenotypic parameters of isolated strains were
studied. The phenotypic characteristics include colony morphology, growth speed,
tolerances to temperature, salt and pH. To assess the genotypic diversity among the isolates, molecular characteristics based on 16S rDNA gene sequencing were performed.
Results: The majority of the isolated strains showed fast-growing capacity (75%). Most
strains tolerate neutral to alkaline pH, however some strains (18%) showed weak growth
capacity at pH 4. All isolates were tolerant to high salt stress ([NaCl] = 3%). The genotypic
characterization based on16S rDNA gene sequencing of the twelve strains showed a high
diversity between the isolates.
Conclusion: Taken together, our results highlight the important biodiversity of the isolated
rhizobial strains and open opportunities for the development of new bio-fertilizer.
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Keywords
Bituminaria bituminosa, Rhizobium, 16S rDNA, symbiosis
Citation
Messaoud Btissam Ben, Aboumerieme Imane, Nassiri Laila, Fahime Elmostafa El, Ibijbijen Jamal. Phenotypic and Genotypic Characteristics of Rhizobia Isolated from Meknes-tafilalet Soils and Study of Their Ability to Nodulate Bituminaria bituminosa. British Microbiology Research Journal. 2014 Apr; 4(4): 405-417.