In vitro Studies on Antimicrobial Activities of Lactic Acid Bacteria Isolated from Fresh Vegetables for Biocontrol of Tomato Pathogens.
Loading...
Date
2014-03
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aims: This study was focused on using Lactic Acid Bacteria (LAB) isolated from fresh
vegetables which has been molecularly identified for in vitro control of some tomato
pathogens.
Study Design: The inhibitory potentials of supernatant obtained from previously
characterized LAB isolates or vegetable origin were investigated against some tomato
phytopathogens using agar-well method with the view to develop biological agents for some
tomato disease causing organisms.
Place and Duration of Study: Biotechnology Centre of Federal University of Agriculture,
Abeokuta, Ogun State, Nigeria, between January 2011 and February 2012.
Methodology: The antimicrobial activities of LAB against some tomato phytopathogenic
bacteria which include (Xanthomonas campestries, Erwinia caratovora, and Pseudomonas
syringae) were obtained by using the agar well diffusion method.
Results: The result indicates that cell free culture of LAB from fresh vegetables origin (Weissella paramesenteroides, Lactobacillus pentosus, Weissella cibaria, Pediococcus
pentosaceus, Weissella kimchi and Lactobacillus plantarum) can inhibits these bacteria by
creating clear zones of inhibition around the wells containing cell free supernatants of the
above mentioned strains of lactic acid bacteria. Pediococcus pentosaceus showed the
highest zone of inhibition against Xanthomonas campestries at 15 mm radius, Weissella
kimchi was the least effective against Pseudomonas syringae at 3.67 mm and Erwinia
caratovora at 3.50 mm radius.
Conclusion: Tomato disease causing organisms can be most likely biologically controlled
by using extracts from LAB. This finding will reduce the potential hazard from the use of
chemical herbicides on plant.
Description
Keywords
Lactic Acid Bacteria, Antimicrobial activities, Pathogens, Bacterioci, Inihibition
Citation
Emerenini E C, Afolabi O R, Okolie P I, Akintokun A K. In vitro Studies on Antimicrobial Activities of Lactic Acid Bacteria Isolated from Fresh Vegetables for Biocontrol of Tomato Pathogens. British Microbiology Research Journal. 2014 Mar; 4(3): 351-359.