In vitro Bioluminescence Used as a Method for Real-Time Inhibition Zone Testing for Antibiotic- Releasing Composites.
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Date
2014-02
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Abstract
Aims: This study describes the potential of real-time bioluminescence imaging in evaluating
the antibiotic efficiency of two cylinder-shaped bioabsorbable antibiotic-releasing
composites by in vitro inhibition zone tests. The bacterial infections of bone tissue can
cause extensive hard and soft tissue damage and decrease the efficiency of oral antibiotic
therapy due to the poor blood circulation in the infected area. To overcome this problem,
new, locally antibiotic-releasing biodegradable composites have been developed.
Study Design & Methodology: The two composites evaluated in this study were
composed of poly(L-lactide-co-ε-caprolactone) matrix, β-tricalcium phosphate ceramic and
either ciprofloxacin or rifampicin antibiotic. The composites were tested with genetically
modified model pathogens of osteomyelitis (Pseudomonas aeruginosa and Staphylococcus
epidermidis) in vitro in inhibition zone tests using a method of real-time bioluminescence.
Results: The first signs of the effect of the released ciprofloxacin or rifampicin became
visible after four hours of incubation and were seen as changed bioluminescence around the composite pellet on a culture dish. Both of the composite types showed excellent effects
against the sensor bacteria within the diffusion area. Bioluminescence measurements
suggested that no survivor bacteria capable of evolving resistant strains were left inside the
inhibition zones. The S. epidermidis bacterial strain was an inhibition sensor and P.
aeruginosa was a stress sensor.
Conclusion: These results highlight the potential of the composite materials against the
pathogens of osteomyelitis. The approach allows continuous visual inspection of the
efficacy of the antibiotics against the bacteria
Description
Keywords
Bioluminescence, ciprofloxacin, controlled drug delivery, inhibition zone, rifampicin
Citation
Mannisto Noora M, Ahola Niina, Karp Matti T, Veiranto Minna, Kellomaki Minna. In vitro Bioluminescence Used as a Method for Real-Time Inhibition Zone Testing for Antibiotic- Releasing Composites. British Microbiology Research Journal. 2014 Feb; 4(2): 235-254.