Sourcing Starter Cultures for Parkia biglobosa Fermentation Part II: Potential of Bacillus subtilis Strains.
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Date
2014-02
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Abstract
Aim: To select good strains of Bacillus subtilis for use as starter culture in the fermentation
of Parkia biglobosa.
Study Design: Fifteen (15) strains of Bacillus subtilis group obtained from commercial
samples were used in starter-culture fermentation of Parkia biglobosa seeds to produce
‘iru’.
Place and Duration of Study: Food Biotechnology Research Unit, National Center for
Genetic Engineering and Biotechnology (BIOTEC), Pahumthani, Thailand, between March
to May 2010. Methodology: The quality of the starter culture-fermented products were compared on the
bases of sensory evaluation, degree of hydrolysis (DH), level of ammonia nitrogen (NH3-N),
pH and enzymatic activities. The 15 strains were also screened for haemolytic activity.
Results: On the basis of the sensory scores of 5 parameters (color, odor, consistency,
texture and over-all liking), particularly the over-all liking, 5 strains were rated the best (in
descending order): BC4333 > 8B > 2B > 7A > 5A, amongst the 15 tested. There were good
correlations between pH and DH (r= 0.926), DH and NH3-N (r=0.962) and between pH and
NH3-N (r=0.945). The strain BC4333 produced the very soft variant of ‘iru’ (‘iru-pete’),
without the addition of ‘kuuru’ (local potash). The quantity of extracellular enzymes
(protease, amylase, pectinase, phytase and lipase) produced during fermentation varied
significantly. None of the 5 strains was haemolytic on sheep blood agar.
Conclusion: The 5 strains of Bacillus subtilis (BC4333, 8B, 2B, 7A, 5A) that showed
potentials of being used as starter cultures for industrial production of ‘iru’, were nonhemolytic
on blood agar.
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Keywords
Bacillus, Parkia biglobosa, starter-culture, haemolytic, enzymes
Citation
Aderibigbe E Y, Visessanguan W, Somphop B, Yutthana K, Jureeporn D. Sourcing Starter Cultures for Parkia biglobosa Fermentation Part II: Potential of Bacillus subtilis Strains. British Microbiology Research Journal. 2014 Feb; 4(2): 224-234.