Distribution of Telomere Length in the Cord Blood of Chinese Newborns.
Loading...
Date
2013-10
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aims: We studied the variability in telomere length in cord blood collected from
newborns of different birth weights and gestational ages.
Study Design: Prospective cohort study.
Place and Duration of Study: Samples were collected from KK Women’s and
Children’s Hospital between March 2011 and March 2012 and the terminal restriction
fragment assays (TRF) were performed at the Department of Physiology, National
University of Singapore.
Methodology: Cord blood samples were prospectively collected in EDTA or heparin
tubes for deliveries from Chinese parents. TRF assays were performed on genomic DNA
extracted from whole blood. Data was collected for birth weight, gestational age, and
maternal age. Variance analyses of telomere lengths and correlation coefficients were
calculated using Statistical Package for the Social Sciences (SPSS). Results: The birth weight of the samples collected ranged from 0.61 kg to 5.18 kg with
gestation age from 196 to 288 days. TRF results from 184 samples (96 males, 88
females) showed that there was a wide range from 6.6 kbp to 19.2 kbp. The mean TRF
length was 12.64 kb (males: 12.33 kb ± 2.50; females: 12.99 kb ± 2.35). There was no
statistically significant correlation of TRF with birth weight, gestation age or maternal
age. There was highly significant correlation of birth weight with gestational age
(P=0.00).
Conclusion: Our results showed no correlation of either gestational age or birth weight
with telomere length as measured by TRF assay.
Description
Keywords
Birth weight, Chinese, gestational age, maternal age, telomere length
Citation
Lim Shi-Ni, Yahya Zaheedah, Zeegers Dimphy, Moe Thiha, Kyaw Ei Ei Phyo, Yeo George SH, Handed M Prakasn, Tan Ene-Choo. Distribution of Telomere Length in the Cord Blood of Chinese Newborns. British Journal of Medicine and Medical Research. 2013 Oct-Dec; 3(4): 1004-1014.