α-Amylase Production on Low-Cost Substrates by Naxibacter sp. Isolated from Mauritian Soils.
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Date
2013-10
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Abstract
Alpha amylase is an important enzyme used in different industries, which degrades starch
into smaller disaccharides. Extracellular α-amylase producing organisms were isolated from
soil samples from Mauritius and identified by standard biochemical tests. In this study, the
high yielding strain was used for amylase production. The potential of four readily available
substrates, namely sugarcane bagasse, potato peel, kitchen wastes and banana peel to
induce amylase production was investigated. Different parameters like temperature (30ºC,
40ºC, 50ºC, 60ºC & 70ºC), different pH (5.0, 6.0, 7.0, 8.0 & 9.0) and inoculum sizes (10%,
20%, 50%, 100% & 150% v/w) were used for the α-amylase production. It was found that
α-amylase production and activity was highest for potato peel at 50ºC at pH 6.0 and
inoculum size 50% (v/w). Amylase assays performed at different incubation temperatures
(30ºC - 60ºC) and pH (5-9) showed that the amylase worked best at 50ºC and pH 7.Based
on results of biochemical tests and 16S ribosomal RNA gene sequences, the isolate was
identified to belong to the Betaproteobacteria, closely related to Naxibacter haematophilus
(99% sequence similarity to the type strain).
Description
Keywords
α-amylase, Naxibacter haematophilus, solid substrate fermentation, Mauritian soils
Citation
Aullybux Aadil Ahmad, Puchooa Daneshwar. α-Amylase Production on Low-Cost Substrates by Naxibacter sp. Isolated from Mauritian Soils. British Microbiology Research Journal. 2013 Oct; 3(4): 478-491.