In vitro Screening of Herbal Extracts and Antibiotics against Bacteria Isolated from Fish Products at Retail Outlets.
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Date
2013-01
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Abstract
Aims: Many studies have been conducted on the antibacterial activity of medicinal plants
against human pathogens. However, a little has been done on fish pathogens. The aim of
this research work was to isolate bacterial pathogens from spoiled fish leading to human
diseases and compare the efficacies of selected antibiotics and medicinal herbal extracts
against these infectious pathogens.
Study Design: An experimental study.
Place and Duration of Study: Biotechnology Lab, Department of Zoology, University of
AJ&K, Muzaffarabad, Pakistan, between Feb 2011 and August 2012.
Methodology: Bacterial pathogens Enterobacter amnigenus, Serratia odorifera,
Salmonella Typhimurium and Shigella flexneri were isolated from spoiled fishes. Various
extracts of seed and stem parts of medicinal plants including Cinnnamomum zylanicum,
Cuminum cyminum, Syzygium aromaticum, Curcuma long Linn, Trachyspermum ammi and
Momordica charantia (both seeds and green parts of Bitter gourd) against common fish associated bacterial pathogens by filter disc diffusion method.
Results: The highest zone of inhibition was observed by Ciprofloxacin against S.
Typhimurium (61 mm), whereas 55 mm by Gentamicin and 51 mm by Streptomycin against
S. flexneri. However, Penicillin G, Ampicillin, and Amoxicillin had no effect on S. flexneri
and E. amnigenus. The extracts of green part of M. charantia showed better results as
compared to the seed extracts. Phytochemical screening of medicinal plants indicated that
individual compounds viz., thyme from ajwain, ar-turmerone from turmeric, eugenol, taninns
and flavonoids from clove have antimicrobial activities.
Conclusion: Current study supports the traditional use of medicinal plants as antibacterial
agents.
Description
Keywords
Antibacterial activity, antibiotics, filter disk diffusion method, medicinal plants, phytochemical analysis
Citation
Ume-Kalsoom, Siddique Sundus, Shahzad Nosheen, Ghous Tahseen, Andleeb Saiqa. In vitro Screening of Herbal Extracts and Antibiotics against Bacteria Isolated from Fish Products at Retail Outlets. British Microbiology Research Journal. 2013 Jan; 3(1): 19-31.