Jordanian Ducrosia flabellifolia Inhibits Proliferation of Breast Cancer Cells by Inducing Apoptosis.
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Date
2013-07
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Abstract
Aims: to evaluate the potential apoptosis inducing effect of Ducrosia flabellifolia extracts
against different cancer cell lines.
Methodology: The antiproliferative activity of Ducrosia flabellifolia extracts was tested
against three cell lines using MTT assay. The apoptosis induction ability of ethanol
extract was determined using TUNEL colorimetric assay while agarose gel
electrophoresis was used to detect DNA fragmentation. Morphological changes
associated with apoptosis were observed using scanning electron microscopy. LC/MSMS
analysis was used to determine the main flavonoids present in the plant extract.
Results: Ducrosia flabellifolia ethanol extract showed selective antiproliferative activity
against different cell lines. The highest activity was against MCF-7 cell line with IC50
value of 25.34 μg/mL, followed by Hep-2 cell line with IC50 value of 98.01 μg/mL. While
the lowest activity was against Vero cell line with IC50 value of 98.01 μg/mL. The antiproliferative effect was exerted by inducing apoptosis as indicted by the presence of
DNA fragmentation, nuclear condensation, and formation of apoptotic bodies in treated
cancer cells. LC/MS-MS analysis revealed the presence of five flavonoids (quercetin,
fisetin, kaempferol, luteolin, and apigenin) and their derivatives in the extract.
Conclusion: The apoptosis inducing ability of Ducrosia flabellifolia ethanol extract
validate the use of this plant in traditional medicine to treat cancer. The anticancer
synergistic effect of Ducrosia flabellifolia compounds has broad implication for
understanding the anticancer potential of plant natural products in vivo, where different
compounds may act in concert to reduce tumor burden.
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Keywords
Flavonoids, anticancer, antiproliferative, plant extract, quercetin
Citation
Talib Wamidh H, Issa Reem A, Kherissat Feryal, Mahasneh Adel M. Jordanian Ducrosia flabellifolia Inhibits Proliferation of Breast Cancer Cells by Inducing Apoptosis. British Journal of Medicine and Medical Research. 2013 Jul-Sep; 3(3): 771-783.