A New Approach to Screening for Methionine-Producing Bacteria.
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Date
2012-01
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Abstract
Aim: To find a method of screening for active Methionine-producing organisms.
Study Design: Examination of cross-section of soil.
Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamdi
Azikiwe University, Awka, Nigeria between April 2010 and August 2011.
Methodology: Bacterial isolates (200) from soil were screened for Methionine producers
on solid agar medium seeded with Methionine auxotroph, Escherichia coli. The agar plates
were observed for halo growth of the E. coli which indicates Methionine production by the
isolate. Methionine production in submerged medium by the isolates was investigated.
Results: A total of 24 bacterial isolates were recovered as Methionine producers. Six of the
active isolates used for submerged fermentation accumulated Methionine in a range of 0.46
– 1.40mg/ml. A close relationship was established between the nature of the halo growths
of E. coli auxotroph on solid agar and the Methionine yields of the active bacterial isolates
in submerged medium.
Conclusion: It is a new and fast approach to screening for active Methionine producers.
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Keywords
Agar medium, bacteria, halo growth, methionine production, soil, submerged medium
Citation
Ozulu U S, Nwanah O U, Ekwealor C C, Dike S K, Nwikpo C L, Ekwealor I A. A New Approach to Screening for Methionine-Producing Bacteria. British Microbiology Research Journal. 2012 Jan; 2(1): 36-39.