Cytotoxicity of Aqueous and Ethanolic Extracts of Ficus deltoidea on Human Ovarian Carcinoma Cell Line.
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Date
2011-10
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Abstract
Aims: This study was to investigate the cytotoxicity of both plant extracts from Ficus
deltoidea (locally known as Mas Cotek), aqueous and ethanolic extracts on human
ovarian carcinoma cells using standard colometric MTT assay.
Study design: Cell based assay
Place and Duration of Study: Institute of Bioproduct Development and Department
of Bioprocess Engineering, Universiti Teknologi Malaysia, Johor Bahru, Malayisa
between January 2007 and December 2009.
Methodology: The biochemical responses of cells after plant sample treatment were
observed and have been reported through several assays such as trypan blue
exclusion assay for cell viability, analysis of glucose uptake and lactate release, cell
survival evaluation and genomic assay through DNA fragmentation.
Results: Both aqueous and ethanolic extracts of the plant sample gave IC50 value of
224.39 + 6.24 μg/ml and 143.03 ± 20.21 μg/ml, respectively. The detachment
capability of the plant aqueous extract was observed in the cell viability assays. DNA
fragmentation was not observed in the aqueous extract, but in ethanolic extract (1000
μg/ml). The DNA was fragmented around 200 Kbp. Morphological observation was
carried out and apoptosis body was observed at 1000 μg/ml of both extract.
Conclusion: A2780 cancer cells behaved differently on cell growth profile upon treating with different concentrations of the aquoues and ethanolic extracts of F.
deltoidea. Even though both extracts could cause apoptosis at 1000 μg/ml, the
aqueous extract prompted to promote cell detachment, and the ethanolic tried to
inhibit cell proliferation through DNA fragmentation.
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Keywords
Ficus deltoidea, human ovarian carcinoma cells, cytotoxicity, DNA fragmentation
Citation
Akhir Nor Azurah Mat, Chua Lee Suan, Majid Fadzilah Adibah Abdul, Sarmidi Mohamad Roji. Cytotoxicity of Aqueous and Ethanolic Extracts of Ficus deltoidea on Human Ovarian Carcinoma Cell Line. British Journal of Medicine and Medical Research. 2011 Oct; 1(4): 397-409.