Drug Resistance in Mycobacterium tuberculosis Isolates from Northeastern Sudan.
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Date
2012-07
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Abstract
The present study intended to determine the drug resistance patterns of Mycobacterium
tuberculosis isolates among human new tuberculosis cases from northeastern Sudan
using conventional and molecular techniques. Of 100 mycobacterial isolates tested by
proportion method, 6% and 2% were identified, respectively, as multi drug resistanttuberculosis
(MDR-TB) and non-multi drug resistant-rifampicin (non-MDR-RIF). A total of
8% was found resistant to rifampicin (RIF), 13% to isoniazid (INH), 34% to streptomycin
(STM) and 12% to ethambutol (EMB). Mono-resistant were 0% for RIF, 1% INH, 18%
STM and 3% ETH. The remaining 61% isolates were identified as sensitive. Resistance
to pyrazinamide was evaluated conventionally for the first time in this country and was
found as low as 1%.
Molecularly, mutations of MDR-TB, RIF, INH, EMB and STM resistant isolates were
detected in 66.7%, 87.5%, 76.9%, 66.7% and 17.6% of corresponding conventional
findings, respectively. The genetic mutations were related to the following codons: rpoB
531 (71.4%) and rpoB 526 (28.6%) for RIF, katG 315 (90%) and mabA-inhA-15 (10%)
for INH, embB 306 (66.7%) for EMB and rpsL (17.6%) for STM.
The study showed that drug resistant tuberculosis increased steadily and provided potentially valuable information on resistant genes circulating in the community. The
rapid solving of this problem can benefit both public health and patient management.
Description
Keywords
Drug, resistance, Mycobacterium, tuberculosis, susceptibility, Sudan
Citation
Hassan S O, Musa M T, Elsheikh H M, Eleragi A M S, Saeed N S. Drug Resistance in Mycobacterium tuberculosis Isolates from Northeastern Sudan. British Journal of Medicine and Medical Research. 2012 Jul-Sep; 2(3): 424-433.