Production of Some Polysaccharides from Kojic Acid Producing Aspergillus oryzae var effusus NRC14 Biomass.
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Date
2014-11
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Abstract
Aims: The present investigation describes the utilization of Aspergillus oryzae var.
effusus NRC14 biomass producing kojic acid in the production of fine chemicals that have
valuable applications such as chitin, chitosan, β-1, 3 glucan and /or their derivatives.
Methodology: Experiments of cell growth and substrate utilization were conducted in
static cultures with identified medium composition. The technology involved in the
extraction of their biopolymers is quite simple and the size scale is compatible with those currently used. This work was performed in Department of Microbial Chemistry, NRC.
Results and Conclusion: The utilization of A. oryzae var. effusus NRC14 biomass waste
actually will cover a part of the cost production of kojic acid; therefore, clean cell walls
preparations were isolated from the fungus biomass at different stages during kojic acid
fermentation in a glucose salts medium. The major chitin constituent was extracted from
the prepared cell walls from six days cultured A. oryzae var. effusus NRC14 biomass by
cold conc. HCl or by 5% of LiCl/ DMAA solvent in yields 4.78% and 2.44%, respectively.
Among the chitin family, chitosan has received special attention since it has several
applications. Chitosan was extracted from alkali insoluble material (cell wall) obtained
from 12 days cultured biomass by using different concentrations of acetic acid (0.5-2.5%)
the results showed that the highest yield of chitosan was 4.65% at a concentration of 2%
acetic acid. The isolated product was characterized by infrared spectrum. Chitosanglucan
complex was isolated in 17.94% yield by treating the A. oryzae var. effusus
NRC14 biomass produced from kojic acid production for 12 days with 40% aqueous
NaOH for 6h. at 95°C. Chitosan-glucan complex was fractionated into its components.
The amount of extracted chitosan was 3.5%; the residue obtained after solubilization of
chitosan by acid treatment was characterized as being mostly insoluble glucan (94%).
Chitin-glucan complex was isolated from 6 days cultured A. oryzae var. effusus NRC14
biomass in yield 17.034%. The lyophilized product was fractionated into its components
(chitin and glucan) in yields 16.36% chitin, 82.72% β, 1-3 glucan. The infrared spectra of
the isolated products as well as of standard samples proved the identity of the isolated
materials as chitosan, chitin and glucan.
Description
Keywords
Asprgillus oryzae var effusus, kojic acid, polysaccharides, chitin, chitosan and glucan
Citation
Saad Abd El-Naby M, Hassan Helmy M, Hazzaa Mahmoud M, Ibrahim Eman I. Production of Some Polysaccharides from Kojic Acid Producing Aspergillus oryzae var effusus NRC14 Biomass. British Biotechnology Journal. 2014 Nov; 4(11): 1212-1222.