In vitro and in silico Approach to Evaluate the Urease and Collagenase Inhibitory Activity of Embilica officinalis Gaertn Fruit.
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Date
2014-10
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Abstract
Aim: The key virulent factors of bacteria are enzymes. Urease and collagenase enzyme
play a vital role in pathogenesis of wide array of bacterial strains and cause numerous
diseases. So the aim of present study was to find out the potent drug candidate from Emblica officinalis Gaertn. fruit for these pathogenically important enzymes.
Study Design: A study was done to screen out the bacteria producing urease and
collagenase from a stack of 19 bacterial strains and the positive strains were checked for
their susceptibility to methanol and ethyl acetate extracts of Emblica officinalis Gaertn.
fruit. Further extracts were investigated for their potential to antagonize these enzymes.
Place and duration of study: Department of Biotechnology KUK, Jwahar Lal University,
Delhi between February 2012 and December 2013.
Methodology: Screening of bacteria and their susceptibility to methanol and ethyl acetate
extracts of E. officinalis was done by using agar diffusion assay. Further investigation of
extracts to antagonize urease and collagenase enzymes was checked by using phenol
hypochlorite and gelatin diffusion assay respectively. GC-MS analysis, docking and
ADME studies were conducted to screen for plant-based urease and collagenase
inhibitors.
Results: Methanol extract inhibited Jack bean urease enzyme (IC50:0.74 mg/ml) more
potently than collagenase Type 1 (IC50:1.13 mg/ml), while ethyl acetate extract inhibited
collagenase completely (IC50:4.19 mg/ml) and was observed to be more effective than
methanol extract (IC50:5.51 mg/ml). GC-MS analysis revealed an array of 28 and 30
compounds in methanol and ethyl acetate extract respectively. In silico study identified
xylenol and erucylamide as active compounds of E. officinalis having good binding score
with better ADME properties compared to standard compounds.
Conclusion: So our observations find application for the consideration of E. officinalis
compounds for further validation towards development of effective drugs against these
significant bacterial enzymes.
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Bai Sheema, Malik Anupma, Seasotiya Leena, Bharti Pooja, Dalal Suita. In vitro and in silico Approach to Evaluate the Urease and Collagenase Inhibitory Activity of Embilica officinalis Gaertn Fruit. British Biotechnology Journal. 2014 Oct; 4(10): 1088-1104.