Imidacloprid Induced Intoxication and its Biodegradation by Soil Isolate Bacillus weihenstephanensis.
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Date
2014-09
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Abstract
Aims: Study was carried out to investigate the effect of imidacloprid on biochemical
parameters and growth of soil isolate. The imidacloprid degradation by the soil isolate was
also studied.
Study Design: The soil isolate was identified and used for toxicity testing. The isolate of
Bacillus weihenstephanensis was further tested for its ability to degrade imidacloprid in
minimal salt medium (MSM) and tryptic soya medium (TSB). The role of plasmid in
imidacloprid degradation was established by curing experiments.
Place and Duration of Study: Department of Biotechnology and Microbiology, Karnatak
University, Dharwad, India between June 2011 and December 2012.
Methodology: The soil isolate was identified by morphological, biochemical characters
and 16s rDNA identification. Effect of imidacloprid on DNA, RNA, protein, glucose and
growth in soil isolate was studied with 10-3 to 10-7 molar imidacloprid for 96 h. Imidacloprid
degradation was determined in MSM and TSB for 28 days with samples taken on 7, 14,
21 and 28th day. The insecticide concentration was tested by HPLC. Plasmid curing was
performed. Results: The soil isolate was identified as Bacillus weihenstephanensis. The study
involving soil isolate Bacillus weihenstephanensis with 10-3 to 10-7 molar imidacloprid
showed significant (P<0.05) decrease in content of DNA, RNA, protein, glucose and
growth. Bacillus weihenstephanensis in MSM and TSB showed 46 and 78 % imidacloprid
degradation in four weeks. The plasmid of Bacillus weihenstephanensis was cured in
fourth generation. 18.80% and 75% degradation observed in cured and non cure cells of
Bacillus weihenstephanensis in TSB.
Conclusion: Study showed that imidacloprid affects the biochemical contents and intern
growth of soil isolate Bacillus weihenstephanensis. Study also revealed that Bacillus
weihenstephanensis was able to degrade imidacloprid in MSM and TSB. Further plasmid
curing revealed that the genes for imidacloprid degradation are located both in plasmid
and chromosome.
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Keywords
Imidacloprid, Bacillus weihenstephanensis, growth, biodegradation
Citation
Shetti A A, Kaliwal R B, Kaliwal B B. Imidacloprid Induced Intoxication and its Biodegradation by Soil Isolate Bacillus weihenstephanensis. British Biotechnology Journal. 2014 Sept; 4(9): 957-969.