Light Microscopic and Immunohistochemical Study Revealed Sex-Dependent Kidney Morphology in DBA/2crSlc Mice.
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Date
2013-07
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Abstract
Aim: Sex-dependent differences in kidney histology have been observed in different
species of the laboratory animals. The present study was conducted to evaluate the sex
and strain-dependent changes in DBA/2CrSlc mouse kidney morphology by using light
microscopy and immunohistochemistry.
Methods: A total of 12 DBA/2CrSlc male and female mice of 2 months of age were used
in this study. Mice were sacrificed by exsanguination under anesthesia using a mixture of
Ketamine and Medetomidine. Both right and left kidneys were removed aseptically and
central slices including hilum were cut perpendicular to the long axis of the organ and
preserved in Zamboni solution. Paraffin blocks were made and tissue sections were
stained with hematoxylin and eosin, and PAS stains to observe the general morphology of
the kidney glomerulus. Immunohistochemistry was performed to detect renin positive sites, expression of Cyclooxygenase-2 (COX-2) and Nitric Oxide Synthase (nNOS).
Number of renin, COX-2 and nNOS positive sites were counted and tabulated. The data
were statistically analyzed for any significant differences between male and female mice.
Results: Our results reveal that the glomerular capsule of male mouse kidney was
consisted of a single layer of simple cuboidal epithelium whereas it was a single layer of
simple squamous epithelium in the female kidney. PAS-positive granules (small and giant
granules) were observed in PST epithelium and collecting ducts in female kidney, but this
feature was absent in male kidneys. Strong nNOS positive reaction for PST epithelium
and collecting ducts was observed in female, but this character was absent in male
kidney. The total number of glomeruli, renin, COX-2, and nNOS positive sites was
comparatively higher in female kidneys then that in male. However, statistical analysis
revealed no significant differences of the areas of renin, nNOS and COX-2-positive sites
between the male and female kidneys (P<0.05).
Conclusion: Light microscopic and immunohistochemical study revealed sex-dependent
histological morphology of the DBA/2CrSlc mouse kidney. DBA/2CrSlc female mouse
kidney revealed renin, COX-2 and nNOS -positive reactions in the present study but male
mice showed nNOS-negative reaction. The reason for nNOS-negative reaction in male is
not clearly understood. It is suggested that this species can be experimentally used in the
laboratory for investigating kidney function and related pathological studies.
Description
Keywords
DBA/2CrSlc mouse, sex dependent, kidney morphology, immunohistochemistry
Citation
Awal M A, Yabuki A, Ichii O, Matsumoto M, Suzuki S, Alam J. Light Microscopic and Immunohistochemical Study Revealed Sex-Dependent Kidney Morphology in DBA/2crSlc Mice. British Biotechnology Journal. 2013 July; 3(3): 341-349.