Domain Analysis and Isolation of Coniferyl Alcohol Dehydrogenase Gene from Pseudomonas nitroreducens Jin1.
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Date
2014-06
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Abstract
Aim: The Aim of present study is to analyse conserved functional Short Dehydrogenase
Reductase (SDR) domain from bacteria. Based on the domain analysis selection of
coniferyl alcohol dehydrogenase gene for isolation from Pseudomonas nitroreducens
Jin1.
Place and Duration of Study: Department of Biotechnology, Punjabi University, Patiala.
From July, 2012 to November, 2012.
Methodology: Bioinformatics tools were used to analyse various calA genes from
bacteria based on the presence of conserved domain in members of SDR family. Based
on insilico analysis, Pseudomonas nitroreducens Jin1 calA was selected. PCR was used
for amplification of the gene from the genome of Pseudomonas nitroreducens Jin1.
Result: Multiple sequence alignment results for conserved domains amongst members of
SDR family identified presence of all domains of Short Dehydrogenase Reductase
members in Pseudomonas nitroreducens Jin1 calA gene. Amongst the various sequences
compared the P. nitroreducens Jin1, calA was found to be the smallest in size. The locus
of calA in the genome resides at 103513-104280 bases. It was amplified from the genome
of Pseudomonas nitroreducens Jin1. The calA gene that was amplified is of size 768bp.
Conclusion: calA gene isolated from Pseudomonas nitroreducens Jin1 is a small gene
with all the functional domains and can be used for biotransformation of coniferyl alcohol
to coniferyl aldehyde.
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Keywords
Coniferyl alcohol dehydrogenase, Pseudomonas nitroreducens Jin1, coniferyl alcohol, coniferyl aldehyde
Citation
Balgir Praveen P, Kalra Dinesh. Domain Analysis and Isolation of Coniferyl Alcohol Dehydrogenase Gene from Pseudomonas nitroreducens Jin1. British Biotechnology Journal. 2014 Jun; 4(6): 684-695.